Cover Image

The injury of serotonin on intestinal epithelium cell renewal of weaned diarrhoea mice

Y. Dong, C. Yang, Z. Wang, Z. Qin, J. Cao, Y. Chen
  • Y. Dong
    China Agricultural University, China
  • C. Yang
    China Agricultural University, China
  • Z. Wang
    China Agricultural University, China
  • Z. Qin
    Shandong Academy of Agricultural Sciences, China
  • J. Cao
    China Agricultural University, China

Abstract

Diarrhoea is a common cause of death in children and weaned animals. Recent research has found that serotonin (5-HT) in the gastrointestinal tract plays an important role in regulating growth and the maintenance of mucosa, which protect against diarrhoea. To determine the influence of 5-HT on intestinal epithelium cell renewal under weaned stress diarrhoea, a weaned-stress diarrhoea mouse model was established with senna infusion (15 mL/Kg) via intragastric administration and stress restraint (SR). Mice with an increase in 5-HT were induced by intraperitoneal injection with citalopram hydrobromide (CH, 10 mg/Kg). The results demonstrated that compared with the control animals, diarrhoea appeared in weaned stress mice and the 5-HT content in the small intestine was significantly increased (P<0.05). Further, the caspase-3 cells and cells undergoing apoptosis in the small intestine were significantly increased, but the VH (villus height), V/C (villus height /crypt depth), and PCNA-positive rate significantly decreased. Compared with the control animals, CH increased the intestinal 5-HT content, caspase-3 cells and cells undergoing apoptosis but decreased the VH and V/C. Compared with both control and weaned stress animals, weaned stress animals that were pre-treated with CH showed higher 5-HT concentrations, positive caspase-3 cells and cells undergoing apoptosis but lower VH, V/C and PCNA-positive rate. In vitro, a low concentration of 5-HT inhibit, IEC-6 cell line apoptosis but a higher concentration of 5-HT promoted it. Therefore, weaned stress diarrhoea mice were accompanied by a 5-HT increase in the small intestine and vice versa, and the increase in 5-HT induced by CH caused diarrhoea. In brief, 5-HT and diarrhoea slowed the intestinal epithelium cell renewal and injured the abortion function and mucosal barrier by decreasing VH, V/C and proliferation and increasing epithelium cell apoptosis.

Keywords

5-HT; weaned stress mice; intestinal epithelium cell; proliferation; apoptosis.

Full Text

PDF
HTML
Submitted: 2016-05-24 13:06:41
Published: 2016-12-28 09:34:08
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
574

Views:
PDF
195
HTML
578

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2016 Yulan Dong, Chenyu Yang, Zixu Wang, Zhuoming Qin, Jing Cao, Yaoxing Chen

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185