Varying effects of EGF, HGF and TGFβ on formation of invadopodia and invasiveness of melanoma cell lines of different origin

Submitted: 21 September 2016
Accepted: 18 November 2016
Published: 9 December 2016
Abstract Views: 1394
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The understanding of melanoma malignancy mechanisms is essential for patient survival, because melanoma is responsible for ca. 75% of deaths related to skin cancers. Enhanced formation of invadopodia and extracellular matrix (ECM) degradation are two important drivers of cell invasion, and actin dynamics facilitate protrusive activity by providing a driving force to push through the ECM. We focused on the influence of epidermal growth factor (EGF), hepatocyte growth factor (HGF) and transforming growth factor β (TGFβ) on melanoma cell invasiveness, since they are observed in the melanoma microenvironment. All three factors stimulated invasion of A375 and WM1341D cells derived from primary tumor sites. In contrast, only EGF and HGF stimulated invasion of WM9 and Hs294T cells isolated from lymph node metastases. Enhanced formation of invadopodia and ECM degradation underlie the increased amount of invasive cells after stimulation with the tested agents. Generally, a rise in invasive potential was accompanied by a decrease in actin polymerization state (F:G ratio). The F:G ratio remained unchanged or was even increased in metastatic cell lines treated with TGFβ. Our findings indicate that the effects of stimulation with EGF, HGF and TGFβ on melanoma cell invasiveness could depend on melanoma cell progression stage. 

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Supporting Agencies

Foundation of Polish Science, project no. HOMING Plus/2010-2/8, National Science Center, project no. 2014/15/B/NZ5/01467,
A. Makowiecka, University of Wrocław, Department of Cell Pathology
PhD student, Department of Cell Pathology
A. Simiczyjew, University of Wrocław, Department of Cell Pathology
Post-Doc, Department of Cell Pathology
D. Nowak, University of Wrocław, Department of Cell Pathology
Head of Department of Cell Pathology
A.J. Mazur, University of Wrocław, Department of Cell Pathology

Principal Investigator, Department of Cell Pathology

How to Cite

Makowiecka, A., Simiczyjew, A., Nowak, D., & Mazur, A. (2016). Varying effects of EGF, HGF and TGFβ on formation of invadopodia and invasiveness of melanoma cell lines of different origin. European Journal of Histochemistry, 60(4). https://doi.org/10.4081/ejh.2016.2728