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Characterization of the role of RILP in cell migration

Azzurra Margiotta, Cinzia Progida, Oddmund Bakke, Cecilia Bucci
  • Azzurra Margiotta
    University of Salento, Department of Biological and Environmental Sciences and Technologies, Italy
  • Cinzia Progida
    University of Oslo, Department of Biosciences, Centre for Immune Regulation, Norway
  • Oddmund Bakke
    University of Oslo, Department of Biosciences, Centre for Immune Regulation, Norway

Abstract

Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration. However, both RILP over-expression and expression of its mutated form, RILP-C33, impair cell adhesion and spreading. In conclusion, our results demonstrate that RILP has important regulatory roles in cell motility affecting migration velocity but also in cell adhesion and cell spreading.

Keywords

RILP; cell migration; cell adhesion; microtubules, cell polarization.

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Submitted: 2017-03-09 16:11:59
Published: 2017-05-31 15:04:40
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Copyright (c) 2017 Azzurra Margiotta, Cinzia Progida, Oddmund Bakke, Cecilia Bucci

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