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Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium

Andrea Ferrigno, Laura G. Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti
  • Andrea Ferrigno
    University of Pavia, Department of Internal Medicine and Therapeutics, Italy
  • Laura G. Di Pasqua
    University of Pavia, Department of Internal Medicine and Therapeutics, Italy
  • Clarissa Berardo
    University of Pavia, Department of Internal Medicine and Therapeutics, Italy
  • Veronica Siciliano
    University of Pavia, Department of Internal Medicine and Therapeutics, Italy
  • Plinio Richelmi
    University of Pavia, Department of Internal Medicine and Therapeutics, Italy

Abstract

The role of Na+ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N2-saturated buffer containing standard (143 mM) or low (25 mM) Na+ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na+, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na+ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na+ buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na+ buffer. Using low-Na+ buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.

Keywords

Liver; hypoxia; Na+; hepatocytes; sinusoidal endothelial cells.

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Submitted: 2017-04-11 14:48:51
Published: 2017-05-30 08:08:02
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Copyright (c) 2017 Andrea Ferrigno, Laura G. Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti

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