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MIAQuant, a novel system for automatic segmentation, measurement, and localization comparison of different biomarkers from serialized histological slices

Elena Casiraghi, Mara Cossa, Veronica Huber, Licia Rivoltini, Matteo Tozzi, Antonello Villa, Barbara Vergani
  • Mara Cossa
    Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
  • Veronica Huber
    Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
  • Licia Rivoltini
    Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
  • Matteo Tozzi
    Insubria University, Italy
  • Barbara Vergani
    University of Milano Bicocca, Italy

Abstract

In the clinical practice, automatic image analysis methods quickly quantizing histological results by objective and replicable methods are getting more and more necessary and widespread. Despite several commercial software products are available for this task, they are very little flexible, and provided as black boxes without modifiable source code. To overcome the aforementioned problems, we employed the commonly used MATLAB platform to develop an automatic method, MIAQuant, for the analysis of histochemical and immunohistochemical images, stained with various methods and acquired by different tools. It automatically extracts and quantifies markers characterized by various colors and shapes; furthermore, it aligns contiguous tissue slices stained by different markers and overlaps them with differing colors for visual comparison of their localization. Application of MIAQuant for clinical research fields, such as oncology and cardiovascular disease studies, has proven its efficacy, robustness and flexibility with respect to various problems; we highlight that, the flexibility of MIAQuant makes it an important tool to be exploited for basic researches where needs are constantly changing. MIAQuant software and its user manual are freely available for clinical studies, pathological research, and diagnosis.

Keywords

Histochemical and immunohistochemical image analysis; digital image processing; statistical analysis; artificial intelligence.

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MIAQuant user Manual
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Submitted: 2017-07-31 10:30:29
Published: 2017-11-02 12:07:35
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Copyright (c) 2017 Elena Casiraghi, Mara Cossa, Veronica Huber, Licia Rivoltini, Matteo Tozzi, Antonello Villa, Barbara Vergani

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