Celecoxib treatment does not alter recruitment and activation of osteoclasts in the initial phase of experimental tooth movement

Submitted: 10 June 2012
Accepted: 10 August 2012
Published: 8 October 2012
Abstract Views: 915
PDF: 492
HTML: 432
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

In a previous study, we reported that the short-term treatment with celecoxib, a non-steroidal anti-inflammatory drug (NSAID) attenuates the activation of brain structures related to nociception and does not interfere with orthodontic incisor separation in rats. The conclusion was that celecoxib could possibly be prescribed for pain in orthodontic patients. However, we did not analyze the effects of this drug in periodontium. The aim of this follow-up study was to analyze effects of celecoxib treatment on recruitment and activation of osteoclasts and alveolar bone resorption after inserting an activated orthodontic appliance between the incisors in our rat model. Twenty rats (400-420 g) were pretreated through oral gavage with celecoxib (50 mg/kg) or vehicle (carboxymethylcellulose 0.4%). After 30 min, they received an activated (30 g) orthodontic appliance, set not to cause any palate disjunction. In sham animals, the appliance was immediately removed after introduction. All animals received ground food and, every 12 h, celecoxib or vehicle. After 48 h, they were anesthetized and transcardiacally perfused through the aorta with 4% formaldehyde. Subsequently, maxillae were removed, post-fixed and processed for histomorphometry or immunohistochemical analyses. As expected, incisor distalization induced an inflammatory response with certain histological changes, including an increase in the number of active osteoclasts at the compression side in group treated with vehicle (appliance: 32.2±2.49 vs sham: 4.8 ± 1.79, P<0.05) and celecoxib (appliance: 31.0±1.45 vs sham: 4.6±1.82, P<0.05). The treatment with celecoxib did not modify substantially the histological alterations and the number of active osteoclasts after activation of orthodontic appliance. Moreover, we did not see any difference between the groups with respect to percentage of bone resorption area. Taken together with our previous results we conclude that short-term treatment with celecoxib can indeed be a therapeutic alternative for pain relieve during orthodontic procedures.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

FAPESP, CNPq
E.P. Carvalho-Filho, Universidade de São Paulo (USP)
Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia de Ribeirão Preto, USP
A.C. Stabile, Universidade de São Paulo (USP)
Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia de Ribeirão Preto, USP
E. Ervolino, Universidade Paulista –Júlio de Mesquita Filho (UNESP)
Departamento de Ciências Básicas, Faculdade de Odontologia de Araçatuba
M.B.S. Stuani, Universidade de São Paulo (USP)
Departamento de Pediatria e Ortodontia, Faculdade de Odontologia de Ribeirão Preto, USP
M.M. Iyomasa, Universidade de São Paulo (USP)
Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia de Ribeirão Preto, USP
M.J.A. Rocha, Universidade de São Paulo
Departamento de Morfologia, Estomatologia e Fisiologia, Faculdade de Odontologia de Ribeirão Preto, USP

How to Cite

Carvalho-Filho, E., Stabile, A., Ervolino, E., Stuani, M., Iyomasa, M., & Rocha, M. (2012). Celecoxib treatment does not alter recruitment and activation of osteoclasts in the initial phase of experimental tooth movement. European Journal of Histochemistry, 56(4), e43. https://doi.org/10.4081/ejh.2012.e43