Expression of collagen type I, II, X and Ki-67 in osteochondroma compared to human growth plate cartilage

  • K Huch | klaus.huch@medizin.uni-ulm.de Department of Orthopaedic Surgery, University of Ulm, Italy.
  • V Mordstein
  • J Stöve
  • AG Nerlich
  • H Arnholdt
  • G Delling
  • W Puhl
  • KP Günther
  • RE Brenner

Abstract

In order to characterize the consequences for the process of endochondral ossification we performed an immunohistochemical study and compared the expression of collagen type I, II and X as markers of cartilage differentiation and Ki-67 as a marker of cell proliferation in solitary (7-26 years, n=9) and multiple (11-42 years, n=6) osteochondromas with their expression in human fetal and postnatal growth plates. In fetal and young postnatal controls, we found a thin superficial layer of articular cartilage that stained positive for collagen type I while collagen II was expressed in the rest of the cartilage and collagen type X was restricted to the hypertrophic zone. Osteochondromas from children showed lobular collagen type II-positive areas surrounded by collagen type I. In adults, the separation of collagen type I- and type II-positive areas was more blurred, or the cartilaginous cap was missing. Collagen type X was detected in a pericellular distribution pattern within hypertrophic zones but also deeper between bone trabecula. The prolif- erative activity of osteochondromas from children younger than 14 years of age was comparable to postnatal growth plates, whereas in cartilage from individuals older than 14 years of age, we could not detect significant proliferative activity.

Downloads

Download data is not yet available.
Published
2010-01-23
Section
Original Papers
Statistics
Abstract views: 505

PDF: 1045
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Huch, K., Mordstein, V., Stöve, J., Nerlich, A., Arnholdt, H., Delling, G., Puhl, W., GüntherK., & Brenner, R. (2010). Expression of collagen type I, II, X and Ki-67 in osteochondroma compared to human growth plate cartilage. European Journal of Histochemistry, 46(3), 249-58. https://doi.org/10.4081/1687