Cellular programming and reprogramming - Methods and protocols

Abstract

There is a great need in contemporary Biology of pluripotent stem cells: for basic researches; now-a-day is just for basic research (from the tests for the pharmacological action of new molecules to the toxicity tests) and tomorrow hopefully for therapeutical purposes. Starting from this simple consideration the reader will immediately catch the relevance of a book that explain in a detailed manner a number of strategies to get these cells in quantity and possibly in well characterized cell lines. The derivation of embryonic pluripotent cells from lefted embryos is constrained by bioethical considerations that in several countries (e.g., Italy and Germany) are turned in restriction laws for researchers..........