Immunohistochemical study of doublecortin and nucleostemin in canine brain

  • E. De Nevi Universitat Autònoma de Barcelona, Spain.
  • P. Marco-Salazar Universitat Autònoma de Barcelona, Spain.
  • D. Fondevila Universitat Autònoma de Barcelona, Spain.
  • E. Blasco Universitat Autònoma de Barcelona, Spain.
  • L. Pérez Universitat Autònoma de Barcelona, Spain.
  • M. Pumarola | eleonora.denevi@gmail.com Universitat Autònoma de Barcelona, Spain.

Abstract

Finding a marker of neural stem cells remains a medical research priority. It was reported that the proteins doublecortin and nucleostemin were related with stem/progenitor cells in central nervous system. The aim of the present immunohistochemical study was to evaluate the expression of these proteins and their pattern of distribution in canine brain, including age-related changes, and in non-nervous tissues. We found that doublecortin had a more specific expression pattern, related with neurogenesis and neuronal migration, while nucleostemin was expressed in most cells of almost every tissue studied. The immunolabeling of both proteins decreased with age. We may conclude that nucleostemin is not a specific marker of stem/progenitor cells in the dog. Doublecortin, however, is not an exclusive marker of neural stem cells, but also of neuronal precursors.

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Author Biographies

E. De Nevi, Universitat Autònoma de Barcelona
Department of Animal Medicine and Surgery
P. Marco-Salazar, Universitat Autònoma de Barcelona
Department of Animal Medicine and Surgery
D. Fondevila, Universitat Autònoma de Barcelona
Department of Animal Medicine and Surgery
E. Blasco, Universitat Autònoma de Barcelona
Department of Animal Medicine and Surgery
L. Pérez, Universitat Autònoma de Barcelona
Center of Animal Biotechnology and Gene Therapy
M. Pumarola, Universitat Autònoma de Barcelona
Department of Animal Medicine and Surgery
Published
2013-03-20
Section
Original Papers
Keywords:
nucleostemin, doublecortin, stem cells, dog brain, aging.
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How to Cite
De Nevi, E., Marco-Salazar, P., Fondevila, D., Blasco, E., PérezL., & Pumarola, M. (2013). Immunohistochemical study of doublecortin and nucleostemin in canine brain. European Journal of Histochemistry, 57(1), e9. https://doi.org/10.4081/ejh.2013.e9