Developmental immunolocalization of the Klotho protein in mouse kidney epithelial cells

  • J.H. Song
  • M.Y. Lee | Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, Deajeon, Korea, Korea, Republic of.
  • Y.J. Kim
  • S.R. Park
  • J. Kim
  • S.Y. Ryu
  • J.Y. Jung


A defect in Klotho gene expression in the mouse results in a syndrome that resembles rapid human aging. In this study, we investigated the detailed distribution and the time of the first appearance of Klotho in developing and adult mouse kidney. Kidneys from 16-(F16), 18-(F18) and 20-day-old (F20) fetuses, 1- (P1), 4- (P4), 7- (P7), 14- (P14), and 21-day-old (P21) pups and adults were processed for immunohistochemistry and immunoblot analyses. In the developing mouse kidney, Klotho immunoreactivity was initially observed in a few cells of the connecting tubules (CNT) of 18-day-old fetus (F) and in the medullary collecting duct (MCD) and distal nephron of the F16 developing kidney. In F20, Klotho immunoreactivity was increased in CNT and additionally observed in the outer portion of MCD and tip of the renal papilla. During the first 3 weeks after birth, Klotho-positive cells gradually disappeared from the MCD due to apoptosis, but remained in the CNT and cortical collecting ducts (CCD). In the adult mouse, the Klotho protein was expressed only in a few cells of the CNT and CCD in cortical area. Also, Klotho immunoreactivity was observed in the aquaporin 2-positive CNT, CCD, and NaCl co-transporter-positive distal convoluted tubule (DCT) cells and type B and nonA-nonB intercalated cells of CNT, DCT, and CCD. Collectively, our data indicate that immunolocalization of Klotho is closely correlated with proliferation in the intercalated cells of CNT and CCD from aging, and may be involved in the regulation of tubular proliferation.


Download data is not yet available.
Original Papers
Supporting Agencies
Korean Goverment (KRF-2006-331-E00009)
Klotho, developing kidney, aging, intercalated cell, tubular proliferation
Abstract views: 2299

PDF: 359
HTML: 500
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Song, J., Lee, M., Kim, Y., Park, S., Kim, J., Ryu, S., & Jung, J. (2014). Developmental immunolocalization of the Klotho protein in mouse kidney epithelial cells. European Journal of Histochemistry, 58(1).