MMP-7 is upregulated by COX-2 and promotes proliferation and invasion of lung adenocarcinoma cells

  • J. Zhang
  • J. Luo
  • J. Ni
  • L. Tang
  • H.P. Zhang
  • L. Zhang
  • J.F. Xu
  • D. Zheng | zhengdiok@aliyun.com Shanghai Pulmonary Hospital, Tongji University School of Medicine,, China.

Abstract

Matrix metalloproteinases (MMPs) have been implicated in a variety of pathophysiological conditions, of which MMP-7 is expressed by tumor cells of epithelial and mesenchymal origin. However, the function of MMP-7 in human lung adenocarcinoma (LAC) is unclear. In the present study the expression of MMP-7 in LAC was examined by immunohistochemical assay using a tissue microarray procedure. A loss-of-function experiment was performed to explore the effects and molecular mechanisms of lentiviral vector-mediated MMP-7 siRNA (siMMP-7) on cell proliferation and invasive potential in LAC A549 cells, measured by MTT and Transwell assays, respectively. It was found that, the expression of MMP-7 protein in LAC was significantly increased compared with that in adjacent non-cancerous tissues (ANCT) (76.0% vs 44.0%, P<0.001), and positively correlated with lymph node metastases of the tumor (P=0.014). Furthermore, targeted inhibition of cyclooxygenase-2 (COX-2) by siRNA downregulated the expression of MMP-7 and inhibited invasion of LAC cells, and knockdown of MMP-7 suppressed tumor proliferation and invasion in LAC cells. Taken together, our findings indicate that increased expression of MMP-7 is associated with lymph node metastasis and upregulated by COX-2, and promotes the tumorigenesis of LAC, suggesting that MMP-7 may be a potential therapeutic target for the treatment of cancer.

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Author Biography

D. Zheng, Shanghai Pulmonary Hospital, Tongji University School of Medicine,
Department of Medical Oncology
Published
2014-02-18
Info
Issue
Section
Original Papers
Supporting Agencies
Shanghai Municipal Natural Science Foundation (No. 10ZR14249000.
Keywords:
MMP-7, COX-2, lung adenocarcinoma
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How to Cite
Zhang, J., Luo, J., Ni, J., Tang, L., Zhang, H., Zhang, L., Xu, J., & Zheng, D. (2014). MMP-7 is upregulated by COX-2 and promotes proliferation and invasion of lung adenocarcinoma cells. European Journal of Histochemistry, 58(1). https://doi.org/10.4081/ejh.2014.2262