Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve

  • A. Arcucci University of Naples Federico II, Italy.
  • M.R. Ruocco University of Naples Federico II, Italy.
  • F. Albano University of Naples Federico II, Italy.
  • G. Granato University of Naples Federico II, Italy.
  • V. Romano University of Naples Federico II, Italy.
  • G. Corso University of Foggia, Italy.
  • C. Bancone Second University of Naples, Italy.
  • E. De Vendittis University of Naples Federico II, Italy.
  • A. Della Corte Second University of Naples, Italy.
  • S. Montagnani | montagna@unina.it University of Naples Federico II, Italy.

Abstract

Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of elastin and collagen fibers. Alterations of extracellular matrix structure and protein composition, typical of medial degeneration, can modulate intracellular pathways. In this study we examined the relevance of superoxide dismutase (SOD3) and Akt in AsAA pathogenesis, evaluating their tissue distribution and protein levels in ascending aortic tissues from controls (n=6), patients affected by AsAA associated to tricuspid aortic valve (TAV, n=9) or bicuspid aortic valve (BAV, n=9). The results showed a significant reduction of SOD3, phospho-Akt and Akt protein levels in AsAA tissues from patients with BAV, compared to controls, whereas the differences observed between controls and patients with TAV  were not significant. The decreased levels of SOD3 and Akt in BAV aortic tissues are associated with decreased Erk1/Erk2 phosphorylation and MMP-9 levels increase. The authors suggest a role of decreased SOD3 protein levels in the progression of AsAA with BAV and a link between ECM modifications of aortic media layer and impaired Erk1/Erk2 and Akt signaling in the late stages of the aortopathy associated with BAV.

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Author Biographies

A. Arcucci, University of Naples Federico II
Department of Public Health
M.R. Ruocco, University of Naples Federico II
Department of Molecular Medicine and Medical Biotechnologies
F. Albano, University of Naples Federico II

Department of Public Health,

Department of Molecular Medicine and Medical Biotechnologies

G. Granato, University of Naples Federico II

Department of Public Health,

Department of Molecular Medicine and Medical Biotechnologies

V. Romano, University of Naples Federico II
Department of Public Health
G. Corso, University of Foggia
Department of Clinical and Experimental Medicine
C. Bancone, Second University of Naples
Department of Cardiothoracic Sciences
E. De Vendittis, University of Naples Federico II
Department of Molecular Medicine and Medical Biotechnologies
A. Della Corte, Second University of Naples
Department of Cardiothoracic Sciences
S. Montagnani, University of Naples Federico II

Department of Public Health,

Full Professor of Human Anatomy

Published
2014-07-18
Info
Issue
Section
Original Papers
Supporting Agencies
Italian Health Ministry “Ministero della Salute, Ricerca Finalizzata” [GR 2009-1580439] - Italian Ministry of University and Research [MIUR-PRIN 2009, prot. 2009P2HZZ7].
Keywords:
Ascending aortic aneurysm, bicuspid aortic valve, medial degeneration, smooth muscle cells, extracellular superoxide dismutase, Akt, tricuspid aortic valve.
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How to Cite
Arcucci, A., Ruocco, M., Albano, F., Granato, G., Romano, V., Corso, G., Bancone, C., De Vendittis, E., Della Corte, A., & Montagnani, S. (2014). Analysis of extracellular superoxide dismutase and Akt in ascending aortic aneurysm with tricuspid or bicuspid aortic valve. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2383

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