Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures

  • H.G. Xu | xuhg@medmail.com.cn Wannan Medical College, China.
  • W. Zhang Wannan Medical College, China.
  • Q. Zheng Wannan Medical College, China.
  • Y.F. Yu Institute of Health Sciences, China.
  • L.F. Deng Institute of Health Sciences, China.
  • H. Wang Wannan Medical College, China.
  • P. Liu Wannan Medical College, China.
  • M. Zhang Wannan Medical College, China.

Abstract

Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced Ank gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of Ank gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and Ank gene expression. We found that ICMC decreased the Ank gene expression in the endplate chondrocytes, and there was an decreased in the  TGF-β1 expression after ICMC stimulation. The Ank gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-β1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of Ank gene expression may be regulated by TGF-β1 in endplate chondrocytes.

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Author Biographies

H.G. Xu, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
W. Zhang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
Q. Zheng, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
Y.F. Yu, Institute of Health Sciences
The Key Laboratory of Stem Cell Biology
L.F. Deng, Institute of Health Sciences
The Key Laboratory of Stem Cell Biology
H. Wang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
P. Liu, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
M. Zhang, Wannan Medical College
Department of Orthopedic Surgery, Yijishan Hospital
Published
2014-07-17
Section
Original Papers
Supporting Agencies
Chinese National Natural Sciences Fund Project, Chinese Anhui Province Natural Science Fund Project
Keywords:
Endplate chondrocytes, ICMC, Ank, TGF-β.
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How to Cite
Xu, H., Zhang, W., Zheng, Q., Yu, Y., Deng, L., Wang, H., Liu, P., & Zhang, M. (2014). Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2415