Expression of the HPV18/E6 oncoprotein induces DNA damage

Submitted: 17 January 2017
Accepted: 30 April 2017
Published: 15 June 2017
Abstract Views: 1280
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Authors

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

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Supporting Agencies

Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).

How to Cite

Cortés Gutiérrez, E. I., Garcí­a-Vielma, C., Aguilar-Lemarroy, A., Vallejo-Ruí­z, V., Piña-Sánchez, P., Zapata-Benavides, P., & Gosalvez, J. (2017). Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2773