Expression of the HPV18/E6 oncoprotein induces DNA damage

  • Elva I. Cortés Gutiérrez | elvacortes@cibinmty.net Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
  • Catalina Garcí­a-Vielma Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Noreste, Mexico.
  • Adriana Aguilar-Lemarroy Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
  • Veronica Vallejo-Ruí­z Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Mexico.
  • Patricia Piña-Sánchez Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Oncológicas, Mexico.
  • Pablo Zapata-Benavides Universidad Autónoma de Nuevo León, Faculty of Biology, Mexico.
  • Jaime Gosalvez Universidad Autónoma de Madrid, Department of Biology, Spain.

Abstract

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

Downloads

Download data is not yet available.
Published
2017-06-15
Section
Original Papers
Supporting Agencies
Fondo de Investigación en Salud-IMSS (FIS/ IMSS/PROT/MD13/1247), Fondo Sectorial de Investigación en Salud y Seguridad Social SS/IMSS/ISSSTE-CONACYT (293539).
Keywords:
DNA damage, DNA breakage detection-fluorescence in situ hybridization, DBD-FISH, human papillomavirus, HPV, E6 oncogene, alkaline-labile sites.
Statistics
Abstract views: 728

PDF: 197
HTML: 190
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Cortés Gutiérrez, E. I., Garcí­a-VielmaC., Aguilar-Lemarroy, A., Vallejo-Ruí­zV., Piña-Sánchez, P., Zapata-Benavides, P., & Gosalvez, J. (2017). Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2773