Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion

  • Marianna Danková Department of Histology and Embryology, Pavol Jozef Šafárik University, Košice, Slovakia.
  • Iveta Domoráková Department of Histology and Embryology, Pavol Jozef Šafárik University, Košice, Slovakia.
  • Zuzana Fagová Department of Histology and Embryology, Pavol Jozef Šafárik University, Košice, Slovakia.
  • Milan Stebnický 2nd Department of Surgery, Pavol Jozef Šafárik University; Louis Pasteur University Hospital, Košice, Slovakia.
  • Alexandra Kunová Department of Histology and Embryology, Pavol Jozef Šafárik University, Košice, Slovakia.
  • Eva Mechírová | eva.mechirova@upjs.sk Department of Histology and Embryology, Pavol Jozef Šafárik University, Košice, Slovakia.

Abstract

The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord segments was used for comparison of antioxidant enzyme levels in sham control, ischemic groups and four preconditioned groups. The total SOD level in the Br or Nor preconditioned groups after 48 h of reperfusion was increased vs Br or Nor preconditioned groups after 24 h of reperfusion. The comparison among the ischemic group vs Br preconditioned (P<0.05), and Nor preconditioned (P<0.001) groups after 48 h of reperfusion, showed statistically significant decrease of Mn-SOD activity. Tissue catalase level activity was significantly decreased in the Br preconditioned group after 48 h of reperfusion (P<0.05) and Nor preconditioned groups after 24 h of reperfusion (P<0.001) and also after 48 h of reperfusion (P<0.001), in comparison to ischemic group after 48 h of reperfusion. Significantly decreased tissue catalase activity (P<0.05) in both Nor preconditioned groups after 24 or 48 h of reperfusion was measured vs Br preconditioned group after 48 h of reperfusion. According to our results, in the white matter, activation of stress proteins in glial cells, as well as antioxidant enzymes levels, were influenced by pharmacological preconditioning followed by 20 min of ischemia and 24 or 48 h of reperfusion. These changes contribute to ischemic tolerance acquisition and tissue protection from oxidative stress during reperfusion period.

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Published
2019-10-18
Section
Original Papers
Supporting Agencies
VEGA grants 1/0348/10, 1/0815/14 and 1/0439/17
Keywords:
Bradykinin, noradrenaline, preconditioning, rabbit, spinal cord, ischemia, antioxidant enzymes
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How to Cite
Danková, M., Domoráková, I., Fagová, Z., Stebnický, M., Kunová, A., & Mechírová, E. (2019). Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion . European Journal of Histochemistry, 63(4). https://doi.org/10.4081/ejh.2019.3045