https://doi.org/10.4081/886

Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results

Vol. 48 No. 2 (2004)
Published: 29 June 2009
Abstract Views: 481
PDF: 611
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Authors

L Benkoël
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P Biagini
F Dodero
X De Lamballerie
P De Micco
A Chamlian
D Lombardo
We localized HCV C-100 protein in liver biopsies of 15 patients with chronic hepatitis C using immunohistochemistry. The results were compared to serum, tissue extract analysis of HCV RNA and in situ RT-PCR described in a previous study. HCV was detected in 80% of the sera tested, in 40% of the tissue extracts and in 80% and 60% of the tissue sections tested by immunohistochemistry and in situ RTPCR respectively. Compared to the serum positive cases, 83% and 67% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR and 41% were positive with tissue extract detection. Compared to the tissue extract positive cases, 25% and 50% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR. Finally, 75% of the cases positive by immunohistochemistry were also positive by in situ RT-PCR. These results underline the complementarity of the different methods for the precise diagnosis of hepatitis C.

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How to Cite

Benkoël, L., Biagini, P., Dodero, F., De Lamballerie, X., De Micco, P., Chamlian, A., & Lombardo, D. (2009). Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results. European Journal of Histochemistry, 48(2), 185–190. https://doi.org/10.4081/886

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