Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix

  • A. Ruggeri | lbreschi@unibs.it
  • G. Orsini
  • A. Mazzoni
  • F. Nato
  • V. Papa
  • M. Piccirilli
  • A. Putignano
  • G. Mazzotti
  • E. De Stefano Dorigo
  • L. Breschi

Abstract

Aim of this study was to investigate the distribution of versican proteoglycan within the human dentine organic matrix by means of a correlative immunohistochemical analysis with field emission in-lens scanning electron microscope (FEI-SEM), transmission electron microscope (TEM), fluorescence microscope (FM) and biochemical assay. Specimens containing dentine and predentine were obtained from non carious human teeth and divided in three groups: 1) FEI-SEM group: sections were exposed to a pre-embedding immunohistochemical procedure; 2) TEM group: specimens were fixed, demineralised, embedded and submitted to a post-embedding immunohistochemical procedure; 3) FM group: sections mineralised and submitted to a pre-embedding immunohistochemical procedure with fluorescence labelling. Specimens were exposed to two different antibodies to assay distribution of versican fragments and whole versican molecule.Western Blotting analysis of dentine and pulp extracts was also performed. The correlative FEI-SEM,TEM and FM analysis revealed positive immunoreaction for versican fragments both in predentine and dentine, while few gold particles identifying the whole versican molecule were found in predentine only under TEM. No labelling of versican whole molecule was detected by FEI-SEM and FM analysis. The immunoblotting analysis confirmed the morphological findings. This study suggests that in fully developed human teeth versican fragments are significant constituents of the human dentine and predentine organic matrix, while versican whole molecule can be visualised in scarce amount within predentine only. The role of versican fragments within human dentine organic matrix should be further elucidated.

Downloads

Download data is not yet available.
Published
2009-09-23
Section
Original Papers
Statistics
Abstract views: 368

PDF: 169
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Ruggeri, A., Orsini, G., Mazzoni, A., Nato, F., Papa, V., Piccirilli, M., Putignano, A., Mazzotti, G., De Stefano Dorigo, E., & Breschi, L. (2009). Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix. European Journal of Histochemistry, 53(3), e15. https://doi.org/10.4081/ejh.2009.e15

Most read articles by the same author(s)