Activation of PKC-e counteracts maturation and apoptosis of HL-60 myeloid leukemic cells in response to TNF family members

Abstract

Protein kinase C (PKC)-e, a component of the serine/threonine PKC family, has been shown to influence the survival and differentiation pathways of normal hematopoietic cells. Here, we have modulated the activity of PKC-e with specific small molecule activator or inhibitor peptides. PKC-e inhibitor and activator peptides showed modest effects on HL-60 maturation when added alone, but PKC-e activator peptide significantly counteracted the pro-maturative activity of tumor necrosis factor (TNF)-a towards the monocytic/macrophagic lineage, as evaluated in terms of CD14 surface expression and morphological analyses. Moreover, while PKC-e inhibitor peptide showed a reproducible increase of TNF-related apoptosis inducing ligand (TRAIL)-induced apoptosis, PKC-e activator peptide potently counteracted the pro-apoptotic activity of TRAIL. Taken together, the anti-maturative and anti-apoptotic activities of PKC-e envision a potentially important proleukemic role of this PKC family member.

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Published
2009-09-23
Section
Original Papers
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How to Cite
Gonelli, A., Milani, D., Rimondi, E., Voltan, R., Grill, V., & Celeghini, C. (2009). Activation of PKC-e counteracts maturation and apoptosis of HL-60 myeloid leukemic cells in response to TNF family members. European Journal of Histochemistry, 53(3), e21. https://doi.org/10.4081/ejh.2009.e21