Activation of PKC-e counteracts maturation and apoptosis of HL-60 myeloid leukemic cells in response to TNF family members

Published: 23 September 2009
Abstract Views: 890
PDF: 696
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Protein kinase C (PKC)-e, a component of the serine/threonine PKC family, has been shown to influence the survival and differentiation pathways of normal hematopoietic cells. Here, we have modulated the activity of PKC-e with specific small molecule activator or inhibitor peptides. PKC-e inhibitor and activator peptides showed modest effects on HL-60 maturation when added alone, but PKC-e activator peptide significantly counteracted the pro-maturative activity of tumor necrosis factor (TNF)-a towards the monocytic/macrophagic lineage, as evaluated in terms of CD14 surface expression and morphological analyses. Moreover, while PKC-e inhibitor peptide showed a reproducible increase of TNF-related apoptosis inducing ligand (TRAIL)-induced apoptosis, PKC-e activator peptide potently counteracted the pro-apoptotic activity of TRAIL. Taken together, the anti-maturative and anti-apoptotic activities of PKC-e envision a potentially important proleukemic role of this PKC family member.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

How to Cite

Gonelli, A., Milani, D., Rimondi, E., Voltan, R., Grill, V., & Celeghini, C. (2009). Activation of PKC-e counteracts maturation and apoptosis of HL-60 myeloid leukemic cells in response to TNF family members. European Journal of Histochemistry, 53(3), e21. https://doi.org/10.4081/ejh.2009.e21