Cytokeratin 20-positive hepatocellular carcinoma

  • D. Fanni |
  • S. Nemolato
  • R. Ganga
  • G. Senes
  • C. Gerosa
  • P. Van Eyken
  • K. Geboes
  • G. Faa


The differential diagnosis between hepatocellular carcinoma (HCC), cholangiocarcinoma (CC) and metastatic colorectal adenocarcinoma (MCA) may be difficult when only based on morphology. For this purpose immunohistochemical analyses are often required, utilizing antibodies directed against CK8- 18, Hep-Par1, glypican 3, CK7, CK19, CK20. Here we report a case of a 65-year-old man who presented with a clinical picture of decompensated cirrhosis. Ultrasonography revealed two nodular areas in the right liver lobe. Liver needle biopsy revealed micro-macronodular cirrhosis associated with HCC with trabecular and pseudoglandular patterns. Immunohistochemically, tumour cells were diffusely positive for CK8- 18 and also diffusely immunostained by glypican 3 and Hep- Par1. Interestingly, a diffuse and strong staining for CK20 was detected in the vast majority of tumor cells, particularly in the areas showing a pseudo-glandular pattern. No immunostaining for CK7 and CK19 was found in the tumor cells. The tumor behaved aggressively, with a rapid diffusion to the whole liver. The patient died from the disease few months after presentation. These findings underline that the interpretation of the expression of CK20 alone in the differential diagnosis among HCC, CC and MCA should be done with caution because a diffuse immunoreactivity for CK20 alone may not rule out the diagnosis of HCC.


Download data is not yet available.
Brief Reports
hepatocellular carcinoma, cholangiocarcinoma, metastatic colorectal carcinomas, CK20.
Abstract views: 524

PDF: 199
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Fanni, D., Nemolato, S., Ganga, R., Senes, G., Gerosa, C., Van Eyken, P., Geboes, K., & Faa, G. (2009). Cytokeratin 20-positive hepatocellular carcinoma. European Journal of Histochemistry, 53(4), e32.

Most read articles by the same author(s)

1 2 > >>