Effect of royal jelly on experimental colitis Induced by acetic acid and alteration of mast cell distribution in the colon of rats

  • T. Karaca | turankaraca@yyu.edu.tr University of Yuzuncu Yil, Fac. Vet. Med., Turkey.
  • F. Bayiroglu University of Yuzuncu Yil, Turkey.
  • M.U. Yoruk University of Yuzuncu Yil, Turkey.
  • S. Kaya University of Yuzuncu Yil, Turkey.
  • S. Uslu University of Yuzuncu Yil, Turkey.
  • B. Comba University of Yuzuncu Yil, Turkey.
  • L. Mis University of Yuzuncu Yil, Turkey.

Abstract

This study investigated the effects of royal jelly (RJ) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg-1 body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg-1 body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 kg-1). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory’s triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RJ was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RJ treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RJ could be used to treat colitis.

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Author Biography

T. Karaca, University of Yuzuncu Yil, Fac. Vet. Med.
Histology-Embryology
Published
2010-10-21
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Section
Original Papers
Keywords:
Royal jell, colitis, mast cell, rat
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How to Cite
Karaca, T., Bayiroglu, F., Yoruk, M., Kaya, S., Uslu, S., Comba, B., & Mis, L. (2010). Effect of royal jelly on experimental colitis Induced by acetic acid and alteration of mast cell distribution in the colon of rats. European Journal of Histochemistry, 54(4), e35. https://doi.org/10.4081/ejh.2010.e35