NADPH-diaphorase expression in the rat jejunum after intestinal ischemia/reperfusion

  • A. Bolekova | a.bolek@seznam.cz Pavol Jozef Safarik University, Slovakia.
  • T. Spakovska Pavol Jozef Safarik University, Slovakia.
  • D. Kluchova Pavol Jozef Safarik University, Slovakia.
  • S. Toth
  • J. Vesela Pavol Jozef Safarik University, Slovakia.

Abstract

The purpose of this study was to analyze the nicotinamide adenine dinucleotide phosphate - diaphorase (NADPH-d) activity in the rat jejunum after a mesenteric ischemia/reperfusion injury. Nitric oxide, synthetised from L-arginine by the enzyme nitric oxide synthase, is a nonadrenergic noncholinergic relaxant neurotransmitter of the intestinal smooth muscle. It plays an important role in the process of plasticity after the ischemia/reperfusion injury. Experimental animals were divided in two groups: the control group and the ischemic/reperfusion group, with different period of the reperfusion. The NADPH-d histochemical method has been used as a marker for the nitric oxide synthase. NADPH-d activity has been rapidly decreased in the neurons of both enteric nervous systems in plexuses of the jejunum after 1 h mesenteric ischemia and 1 h reperfusion. Differences were predominantly detected in the myenteric plexus; they were seen in change of the neuronal shape, in the arrangement of neurons and in intensity of their staining. The NADPH-d positivity was absent in the intestinal crypts. After 1 h ischemia and 24 h reperfusion, the NADPH-d activity was gradually increased, but it was lower in comparison with the control group. On the 30th day following the ischemia/reperfusion there were no changes in NADPH-d positivity compared with the control animals. These results indicated that the jejunal ischemia/reperfusion has affected the neurons of the enteric nervous system of adult rats and resulted in the early decrease of NADPH-d positivity 1 h of the reperfusion insult. The gradual increasing of NADPH-d activity in 24 h following the reperfusion could be considered as a result of the plasticity process. On the 30th day after the ischemia/reperfusion all histochemical changes were returned to the control levels.

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Author Biographies

A. Bolekova, Pavol Jozef Safarik University
Department of Anatomy, Faculty of Medicine
T. Spakovska, Pavol Jozef Safarik University
Department of Anatomy, Faculty of Medicine
D. Kluchova, Pavol Jozef Safarik University
Department of Anatomy, Faculty of Medicine
S. Toth
Department of Histology and Embryology, Faculty of Medicine
J. Vesela, Pavol Jozef Safarik University
Department of Histology and Embryology, Faculty of Medicine
Published
2011-07-13
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Original Papers
Supporting Agencies
this work was supported by Grant no. APVV-0252-07.
Keywords:
jejunum, NADPH-d, histochemistry, nitric oxide synthase, ischemia/reperfusion
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How to Cite
Bolekova, A., Spakovska, T., Kluchova, D., Toth, S., & Vesela, J. (2011). NADPH-diaphorase expression in the rat jejunum after intestinal ischemia/reperfusion. European Journal of Histochemistry, 55(3), e23. https://doi.org/10.4081/ejh.2011.e23