Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank

  • C. Bai Chinese Academy of Agricultural Sciences, China.
  • D. Wang Chinese PLA General Hospital, China.
  • C. Li Northeast Forestry University, China.
  • D. Jin Chinese Academy of Agricultural Sciences, China.
  • C. Li Chinese Academy of Agricultural Sciences, China.
  • W. Guan Chinese Academy of Agricultural Sciences, China.
  • Y. Ma | yuehui_ma@hotmail.com Chinese Academy of Agricultural Sciences, China.

Abstract

Using tissue explantation and cryopreservation biotechniques, a Jingning chicken embryonic fibroblast bank was successfully established, which includes 43 embryo samples and a stock of 178 cryovials, each one containing 3.0×106 cells. Most of the cells were apparently fibroblasts in their morphology, and the population doubling time (PDT) was about 48 h. The total chromosome number of a diploid cell was 78. According to karyotyping and G-banding, the diploid rate in the cell bank was 97.62±2.12%. The cells were tested for microbial contamination and found free of infections from bacteria, fungi, viruses and mycoplasms. There was no cross-contamination from other cell lines as revealed by lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzyme polymorphisms. Six fluorescent proteins were transfected into the Jingning chicken embryonic fibroblasts, and the transfection efficiencies of these genes were between 10.1 and 41.9%. All the tests showed that the quality of the cell line conforms to the quality criteria of the ATCC (American type culture collection). This work succeeded not only in preserving the genetic resources of Jingning chicken, but it also established a new protocol to preserve endangered animal breeds.

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Author Biographies

C. Bai, Chinese Academy of Agricultural Sciences
Institute of Animal Sciences
D. Wang, Chinese PLA General Hospital
Department of Pathology
D. Jin, Chinese Academy of Agricultural Sciences
Institute of Animal Sciences
W. Guan, Chinese Academy of Agricultural Sciences
Institute of Animal Sciences
Y. Ma, Chinese Academy of Agricultural Sciences
Institute of Animal Sciences
Published
2011-03-29
Section
Original Papers
Supporting Agencies
This research was supported by the “863” National Major Research Program (2006AA10Z198, 2007AA10Z170), National Key Technology R&D Program (2006BAD13B08, 2008BADB2B01), National Scientific Foundation of China (30671539) and Project (no. 2008ZX08009-003) f
Keywords:
Jingning Chicken, Fibroblasts, Genetic conservation, Biological characteristics
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How to Cite
Bai, C., Wang, D., Li, C., Jin, D., Li, C., Guan, W., & Ma, Y. (2011). Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank. European Journal of Histochemistry, 55(1), e4. https://doi.org/10.4081/ejh.2011.e4