Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats

  • Y. Zhu
  • D. Ning
  • F. Wang
  • C. Liu
  • Y. Xu
  • X. Jia
  • D. Zhu | zhudfcn@yeah.net The First Affiliated Hospital of Anhui Medical University, China.

Abstract

Adult-onset hypothyroidism induces a variety of impairments on hippocampus- dependent neurocognitive functioningin which many synaptic proteins in hippocampus neurons are involved. Here, we observed the effect of adult-onset hypothyroidism on the expression of syntaxin-1 and munc-18 in the dorsal hippocampus and whether the altered proteins could be restored by levothyroxine (T4) treatment. All rats were separated into 4 groups randomly: hypothyroid group, 5μg T4/100 g body weight (BW) treated group, 20 μg T4/100g BW treated group and control group. The radioimmunoassay kits were applied to assay the levels of serum T3 and T4, and the levels of syntaxin-1 and munc-18 in hippocampus were assessed by immunohistochemistry and Western blot. Both analysis corroborated that syntaxin-1 in the hypothyroid group was significantly higher. Munc-18 was lower in four layers of CA3 and dentate gyrus by immunohistochemistry. After two weeks of treatment with 5 μg T4/100g BW for hypothyroidism, syntaxin-1 levels were completely restored, whereas the recovery of munc-18 only located in two of the four impaired layers. Twenty μg T4/100g BW treatment normalized munc-18 levels. These data suggested that adult-onset hypothyroidism induced increment of syntaxin-1 and decrement of munc-18 in the dorsal hippocampus, which could be restored by T4 treatment. Larger dosage of T4 caused more effective restorations.

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Published
2012-05-22
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Original Papers
Supporting Agencies
This study was supported by the National Natural Science Foundation of China (30772301)
Keywords:
hypothyroidism, levothyroxine (T4), munc-18, syntaxin-1, hippocampus
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How to Cite
Zhu, Y., Ning, D., Wang, F., Liu, C., Xu, Y., Jia, X., & Zhu, D. (2012). Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats. European Journal of Histochemistry, 56(2), e22. https://doi.org/10.4081/ejh.2012.22