CM2 antigen, a potential novel molecule participating in glucuronide transport on rat hepatocyte canalicular membrane

  • L. Wang The Fourth Military Medical University, China.
  • J. Wang The Fourth Military Medical University, China.
  • X. Zhou The Fourth Military Medical University, China.
  • J. Li The Fourth Military Medical University, China.
  • Y. Shi The Fourth Military Medical University, China.
  • Z. Han The Fourth Military Medical University, China.
  • X. Wang The Fourth Military Medical University, China.
  • S. Li The Fourth Military Medical University, China.
  • Z. Yang The Fourth Military Medical University, China.
  • R. Wang The Fourth Military Medical University, China.
  • D. Fan The Fourth Military Medical University, China.
  • Y. Han | happylu029@163.com The Fourth Military Medical University, China.

Abstract

The polarized molecules predominately distributing at hepatocyte canalicular surface play a vital role in disclosing the process of bile formation and etiopathogenisis of cholestatic live diseases. Therefore, it is important to find novel polarized molecules on hepatocyte canalicular membrane. In the present study, canalicular membrane vesicles (CMVs) isolated from rat hepatocyte by density gradient centrifugation were used as immunogens to produce hybridoma and 46 strains of monoclonal antibodies (mAb) against CMVs were obtained. With a series of morphological assay methods, including immunohistochemistry, immunofluorescence and immuno-electron microscope, the antigens recognized by canalicular mAb1 (CM1) and canalicular mAb2 (CM2) were confirmed to predominately distribute at hepatocyte canalicular membrane. Transport activity assay revealed that CM2 could inhibit ATP-dependent E217βG uptake of rat hepatocyte CMVs. Meanwhile, Western blotting analysis showed that the molecular mass of CM2 antigen was approximately 110kDa, which was much less than Mr 180kDa of multidrug resistance-associated protein 2 (MRP2) involved in glucuronide transport. These data indicated that CM2 antigen might be a potential novel molecule participating in glucuronide transport on the hepatocyte canalicular membrane.

Downloads

Download data is not yet available.

Author Biographies

J. Wang, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
X. Zhou, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
J. Li, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
Y. Shi, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
Z. Han, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
X. Wang, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
S. Li, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
Z. Yang, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
R. Wang, The Fourth Military Medical University
Department of Pathology
D. Fan, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
Y. Han, The Fourth Military Medical University
Division of Hepatology, Xijing Hospital of Digestive Diseases
Published
2012-06-29
Section
Original Papers
Supporting Agencies
this study was supported by the National Natural Science Foundation of China (NO. 30971339, 30971116, 81071873 and 81070326).
Keywords:
hepatocyte canalicular membrane, glucuronide transport, canalicular mAb2 (CM2), hybridoma technique
Statistics
Abstract views: 412

PDF: 156
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Wang, L., Wang, J., Zhou, X., Li, J., Shi, Y., Han, Z., Wang, X., Li, S., Yang, Z., Wang, R., Fan, D., & Han, Y. (2012). CM2 antigen, a potential novel molecule participating in glucuronide transport on rat hepatocyte canalicular membrane. European Journal of Histochemistry, 56(3), e26. https://doi.org/10.4081/ejh.2012.e26