Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis

  • J.P. Damico University of São Paulo, Brazil.
  • E. Ervolino Univ Estadual Paulista, Brazil.
  • K.R. Torres University of São Paulo, Brazil.
  • D.S. Batagello University of São Paulo, Brazil.
  • R.J. Cruz-Rizzolo Univ Estadual Paulista, Brazil.
  • C.A. Casatti | ccasatti@foa.unesp.br University of São Paulo, Brazil.
  • J.A. Bauer University of São Paulo, Brazil.

Abstract

The aim of this study was to identify immunoreactive neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) neurons in the autonomic and sensory ganglia, specifically neurons that innervate the rat temporomandibular joint (TMJ). A possible variation between the percentages of these neurons in acute and chronic phases of carrageenan-induced arthritis was examined. Retrograde neuronal tracing was combined with indirect immunofluorescence to identify NPY-immunoreactive (NPY-IR) and CGRP- immunoreactive (CGRP-IR) neurons that send nerve fibers to the normal and arthritic temporomandibular joint. In normal joints, NPY-IR neurons constitute 78±3%, 77±6% and 10±4% of double-labeled nucleated neuronal profile originated from the superior cervical, stellate and otic ganglia, respectively. These percentages in the autonomic ganglia were significantly decreased in acute (58±2% to superior cervical ganglion and 58±8% to stellate ganglion) and chronic (60±2% to superior cervical ganglion and 59±15% to stellate ganglion) phases of arthritis, while in the otic ganglion these percentages were significantly increased to 19±5% and 13±3%, respectively. In the trigeminal ganglion, CGRP-IR neurons innervating the joint significantly increased from 31±3% in normal animals to 54±2% and 49±3% in the acute and chronic phases of arthritis, respectively. It can be concluded that NPY neurons that send nerve fibers to the rat temporomandibular joint are located mainly in the superior cervical, stellate and otic ganglia. Acute and chronic phases of carrageenan-induced arthritis lead to an increase in the percentage of NPY-IR parasympathetic and CGRP-IR sensory neurons and decrease in the percentage of NPY-IR sympathetic neurons related to TMJ innervation.

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Author Biographies

J.P. Damico, University of São Paulo
Department of Cell and Developmental Biology, Institute of Biomedical Sciences
E. Ervolino, Univ Estadual Paulista
Department of Basic Sciences, School of Dentistry of Araçatuba
K.R. Torres, University of São Paulo
Department of Anatomy, Institute of Biomedical Sciences
D.S. Batagello, University of São Paulo
Department of Anatomy, Institute of Biomedical Sciences
R.J. Cruz-Rizzolo, Univ Estadual Paulista
Department of Basic Sciences, School of Dentistry of Araçatuba
C.A. Casatti, University of São Paulo
Departament of Basic Sciences
J.A. Bauer, University of São Paulo
Department of Cell and Developmental Biology, Institute of Biomedical Sciences
Published
2012-10-01
Section
Original Papers
Supporting Agencies
FAPESP (The State of São Paulo Research Foundation, SP, Brasil), fellowship grant from CAPES (the Federal Agency of Support and Evaluation of Postgraduate Education, Brasil).
Keywords:
trigeminal ganglion, otic ganglion, superior cervical ganglion, arthritis, temporomandibular joint
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How to Cite
Damico, J., Ervolino, E., Torres, K., Batagello, D., Cruz-Rizzolo, R., Casatti, C., & Bauer, J. (2012). Phenotypic alterations of neuropeptide Y and calcitonin gene-related peptide-containing neurons innervating the rat temporomandibular joint during carrageenan-induced arthritis. European Journal of Histochemistry, 56(3), e31. https://doi.org/10.4081/ejh.2012.e31