Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)

  • A. Amaroli | amaroli@dipteris.unige.it DIPTERIS - Università degli Studi di Genova, Italy.
  • M.G. Chessa DIPTERIS - Università degli Studi di Genova, Italy.

Abstract

In Dictyostelium discoideum (D. discoideum), compounds generating nitric oxide (NO) inhibit its aggregation and differentiation without altering cyclic guanosine monophosphate (cGMP) production. They do it by preventing initiation of cyclic adenosine monophosphate (cAMP) pulses. Furthermore, these compounds stimulate adenosine diphosphate (ADP)-ribosylation of a 41 kDa cytosolic protein and regulate the glyceraldehyde-3-phospate dehydrogenase activity. Yet, although D. discoideum cells produce NO at a relatively constant rate at the onset of their developmental cycle, there is still no evidence of the presence of nitric oxide synthase (NOS) enzymes. In this work, we detect the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity in D. discoideum and we characterise it by specific inhibitors and physical-chemical conditions that allegedly distinguish between NOS-related and -unrelated NADPH-d activity.

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Published
2012-10-29
Section
Original Papers
Keywords:
NADPH-diaphorase activity, protozoa, nitric oxide synthase
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How to Cite
Amaroli, A., & Chessa, M. (2012). Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa). European Journal of Histochemistry, 56(4), e47. https://doi.org/10.4081/ejh.2012.e47