DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells

  • E. I. Cortés-Gutiérrez | elvacortes@cibinmty.net Instituto Mexicano del seguro Social, Mexico.
  • M. I. Davila-Rodríguez
  • J. L. Fernández
  • C. López-Fernández
  • J. Gosalvez

Abstract

DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

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Published
2012-12-28
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Section
Technical Notes
Keywords:
DNA damage, buccal cell, DNA breakage detection-fluorescence in situ hybridization, DBD-FISH
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How to Cite
Cortés-GutiérrezE. I., Davila-RodríguezM. I., Fernández, J. L., López-FernándezC., & Gosalvez, J. (2012). DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells. European Journal of Histochemistry, 56(4), e49. https://doi.org/10.4081/ejh.2012.e49

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