DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells
AbstractDNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.
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Copyright (c) 2012 E. I. CortÃ©s-GutiÃ©rrez, M. I. Davila-RodrÃguez, J. L. FernÃ¡ndez, C. LÃ³pez-FernÃ¡ndez, J. Gosalvez
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