Endothelial barrier antigen-immunoreactivity is conversely associated with blood-brain barrier dysfunction after embolic stroke in rats

  • J. Pelz University of Leipzig, Germany.
  • W. Härtig University of Leipzig, Germany.
  • C. Weise University of Leipzig, Germany.
  • C. Hobohm University of Leipzig, Germany.
  • D. Schneider University of Leipzig, Germany.
  • M. Krueger University of Leipzig, Germany.
  • J. Kacza University of Leipzig, Germany.
  • D. Michalski | dominik.michalski@medizin.uni-leipzig.de University of Leipzig, Germany.

Abstract

While the concept of the Neurovascular Unit (NVU) is increasingly recognized for exploring mechanisms of tissue damage in ischemic stroke, immunohistochemical analyses are of interest to specifically visualize constituents like the endothelium. Changes in immunoreactivity have also been discussed to reflect functional aspects, e.g., the integrity of the blood-brain barrier (BBB). This study aimed to characterize the endothelial barrier antigen (EBA) as addressed by the antibody SMI-71 in a rat model of embolic stroke, considering FITC-albumin as BBB leakage marker and serum levels of BBB-associated matrix metalloproteinases (MMPs) to explore its functional significance. Five and 25 h after ischemia onset, regions with decreased BBB integrity exhibited a reduction in number and area of EBA-immunopositive vessels, while the stained area per vessel was not affected. Surprisingly, EBA content of remaining vessels tended to be increased in areas of BBB dysfunction. Analyses addressing this interrelation resulted in a significant and inverse correlation between the vessels’ EBA content and degree of BBB permeability. In conclusion, these data provide evidence for a functional relationship between EBA-immunoreactivity and BBB dysfunction in experimental ischemic stroke. Further studies are required to explore the underlying mechanisms of altered EBA-immunoreactivity, which might help to identify novel neuroprotective strategies.

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Author Biographies

J. Pelz, University of Leipzig
Department of Neurology
W. Härtig, University of Leipzig
Paul Flechsig Institute for Brain Research
C. Weise, University of Leipzig
Department of Neurology
C. Hobohm, University of Leipzig
Department of Neurology
D. Schneider, University of Leipzig
Department of Neurology
M. Krueger, University of Leipzig
Institute of Anatomy
J. Kacza, University of Leipzig
Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine
D. Michalski, University of Leipzig
Department of Neurology
Published
2013-12-18
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Original Papers
Keywords:
ischemic stroke, embolic model, EBA, blood-brain barrier, matrix metalloproteinase.
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How to Cite
Pelz, J., HärtigW., Weise, C., Hobohm, C., Schneider, D., Krueger, M., Kacza, J., & Michalski, D. (2013). Endothelial barrier antigen-immunoreactivity is conversely associated with blood-brain barrier dysfunction after embolic stroke in rats. European Journal of Histochemistry, 57(4), e38. https://doi.org/10.4081/ejh.2013.e38