An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

  • S. Shibata | sshibata.mfa@tmd.ac.jp Tokyo Medical and Dental University, Japan.
  • Y. Sakamoto Tokyo Medical and Dental University, Japan.
  • O. Baba Ohu University School of Dentistry, Japan.
  • C. Qin Texas A & M University, Baylor College of Dentistry, System Health Science Center, United States.
  • G. Murakami Iwamizawa Koujin-kai Hospital, Japan.
  • B.H. Cho Chonbuk National University, Korea, Republic of.

Abstract

Immunohistochemical localization of collagen types I, II, and X, aggrecan, versican, dentin matrix protein (DMP)-1, martix extracellular phosphoprotein (MEPE) were performed for Meckel’s cartilage, cranial base cartilage, and mandibular condylar cartilage in human midterm fetuses; staining patterns within the condylar cartilage were compared to those within other cartilaginous structures. Mandibular condylar cartilage contained aggrecan; it also had more type I collagen and a thicker hypertrophic cell layer than the other two types of cartilage; these three characteristics are similar to those of the secondary cartilage of rodents. MEPE immunoreactivity was first evident in the cartilage matrix of all types of cartilage in the human fetuses and in Meckel’s cartilage of mice and rats. MEPE immunoreactivity was enhanced in the deep layer of the hypertrophic cell layer and in the cartilaginous core of the bone trabeculae in the primary spongiosa. These results indicated that MEPE is a component of cartilage matrix and may be involved in cartilage mineralization. DMP-1 immunoreactivity first became evident in human bone lacunae walls and canaliculi; this pattern of expression was comparable to the pattern seen in rodents. In addition, chondroid bone was evident in the mandibular (glenoid) fossa of the temporal bone, and it had aggrecan, collagen types I and X, MEPE, and DMP-1 immunoreactivity; these findings indicated that chondroid bone in this region has phenotypic expression indicative of both hypertrophic chondrocytes and osteocytes.

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Author Biographies

S. Shibata, Tokyo Medical and Dental University
Maxillofacial Anatomy, Department of Maxillofacial Biology, Graduate School
Y. Sakamoto, Tokyo Medical and Dental University
Basic Sciences of Oral Health Care, Graduate School
O. Baba, Ohu University School of Dentistry
Oral Function and Molecular Biology
C. Qin, Texas A & M University, Baylor College of Dentistry, System Health Science Center
Department of Biomedical Sciences
G. Murakami, Iwamizawa Koujin-kai Hospital
Division of Internal Medicine
B.H. Cho, Chonbuk National University
Department of Surgery, Faculty of Medicine
Published
2013-12-02
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Original Papers
Supporting Agencies
this work was supported by Grant-in-Aid for Scientific Research (No. 22592044) from Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Keywords:
condylar cartilage, human fetus, extracellular matrix, MEPE, DMP-1.
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How to Cite
Shibata, S., Sakamoto, Y., Baba, O., Qin, C., Murakami, G., & Cho, B. (2013). An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses. European Journal of Histochemistry, 57(4), e39. https://doi.org/10.4081/ejh.2013.e39