Expression of 8-OHdG in Zosterisessor ophiocephalus from the Venetian lagoon, Italy

Submitted: 26 September 2012
Accepted: 5 December 2012
Published: 19 February 2013
Abstract Views: 1071
PDF: 625
HTML: 674
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

The aim of the present work was to evaluate the expression of 8-OHdG (8-hydroxydeoxyguanosine) in the benthic fish Zosterisessor ophiocephalus collected in two differently polluted sites of the Venetian lagoon (Porto Marghera and Caroman). We compared our data on 8-OHdG with those of CYP1A (Cytochrome P450, family 1, subfamily A, polypeptide 1), which is a well known biomarker for detoxification of contaminants. Immunohistochemistry with an antibody to 8-OHdG showed immunopositivity in nuclei of hepatocytes as well as in melanomacrophage centres of spleen and kidney, whereas an anti-CYP1A antibody exhibited positive immunostaining in the liver, kidney and ovary. The liver of males showed higher expression of both proteins than females. In animals from Porto Marghera site, the enzymatic assay for 8-OHdG exhibited higher levels in liver of males than in females. Western Blot analysis using the antibody anti-CYP1A recognized the presence of a band of about 60 kDa in the liver of males and females. Males exhibited a strong band, whereas in females the band showed a lower intensity. By using Real-Time PCR, the mRNA expression of CYP1A did not show any differences between males and females from each site, but it was at borderline significance level. Comparing the two sites, mRNA expression of CYP1A was significantly higher in the liver of both males and females from Porto Marghera than that of Caroman. The present data suggest that pollutants are bio-available as demonstrated by our biomarker analyses and may have a harmful effect on aquatic organisms such as Z. ophiocephalus. We report that the highest levels of hepatic 8-OHdG and CYP1A expression were detected in males, showing clear gender specificity.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

Veneto Region
G. Radaelli, Dipartimento di Biomedicina Comparata e Alimentazione, Università di Padova
Dipartimento di Biomedicina Comparata e Alimentazione, Università di Padova

How to Cite

Negrato, E., Vascellari, M., Capolongo, F., Binato, G., Da Dalt, L., Boscolo Papo, M., … Pascoli, F. (2013). Expression of 8-OHdG in Zosterisessor ophiocephalus from the Venetian lagoon, Italy. European Journal of Histochemistry, 57(1), e8. https://doi.org/10.4081/ejh.2013.e8

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Publication Facts

Metric
This article
Other articles
Peer reviewers 
3
2.4

Reviewer profiles  N/A

Author statements

Author statements
This article
Other articles
Data availability 
N/A
16%
External funding 
N/A
32%
Competing interests 
Yes
11%
Metric
This journal
Other journals
Articles accepted 
57%
33%
Days to publication 
145
145

Indexed in

Editor & editorial board
profiles
Academic society 
N/A