@article{Malatesta_Zancanaro_Costanzo_Cisterna_Pellicciari_2013, title={Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabeling in cultured cells}, volume={57}, url={https://www.ejh.it/ejh/article/view/ejh.2013.e26}, DOI={10.4081/ejh.2013.e26}, abstractNote={Diaminobenzidine photoconversion is a technique by which a fluorescent dye is transformed into a stably insoluble, brown, electrondense signal, thus enabling examination at both bright field light microscopy and transmission electron microscopy. In this work, a procedure is proposed for combining photoconversion and immunoelectron microscopy: <em>in vitro</em> cell cultures have been first submitted to photoconversion to analyse the intracellular fate of either fluorescent nanoparticles or photosensitizing molecules, then processed for transmission electron microscopy; different fixative solutions and embedding media have been used, and the ultrathin sections were finally submitted to post-embedding immunogold cytochemistry. Under all conditions the photoconversion reaction product and the target antigen were properly detected in the same section; Epon-embedded, osmicated samples required a pre-treatment with sodium metaperiodate to unmask the antigenic sites. This simple and reliable procedure exploits a single sample to simultaneously localise the photoconversion product and a variety of antigens allowing a specific identification of subcellular organelles at the ultrastructural level.}, number={3}, journal={European Journal of Histochemistry}, author={Malatesta, M. and Zancanaro, C. and Costanzo, M. and Cisterna, B. and Pellicciari, C.}, year={2013}, month={Sep.}, pages={e26} }