@article{Ayarza_González_López_Fernández-Donoso_Page_Berrios_2016, title={Alterations in chromosomal synapses and DNA repair in apoptotic spermatocytes of Mus m. domesticus}, volume={60}, url={https://www.ejh.it/ejh/article/view/2677}, DOI={10.4081/ejh.2016.2677}, abstractNote={<p>We investigated whether apoptotic spermatocytes from the mouse <em>Mus m. domesticus</em> presented alterations in chromosomal synapses and DNA repair. To enrich for apoptotic spermatocytes, the scrotum’s temperature was raised by partially exposing animals for 15 min to a 42ºC water bath. Spermatocytes in initial apoptosis were identified <em>in situ</em> by detecting activated Caspase-9.  SYCP1 and SYCP3 were markers for evaluating synapses or the structure of synaptonemal complexes and Rad51 and γH2AX for detecting DNA repair and chromatin remodeling. Apoptotic spermatocytes were concentrated in spermatogenic cycle stages III-IV (50.3%), XI-XII (44.1%) and IX-X (4.2%). Among apoptotic spermatocytes, 48% were in middle pachytene, 44% in metaphase and 6% in diplotene. Moreover, apoptotic spermatocytes showed several structural anomalies in autosomal bivalents, including splitting of chromosomal axes and partial asynapses between homologous chromosomes. γH2AX and Rad51 were atypically distributed during pachytene and as late as diplotene and associated with asynaptic chromatin, single chromosome axes or discontinuous chromosome axes. Among apoptotic spermatocytes at pachytene, 70% showed changes in the structure of synapses, 67% showed changes in γH2AX and Rad51 distribution and 50% shared alterations in both synapses and DNA repair. Our results showed that apoptotic spermatocytes from <em>Mus m. domesticus</em> contain a high frequency of alterations in chromosomal synapses and in the recruitment and distribution of DNA repair proteins. Together, these observations suggest that these alterations may have been detected by meiotic checkpoints triggering apoptosis.</p>}, number={2}, journal={European Journal of Histochemistry}, author={Ayarza, E. and González, M. and López, F. and Fernández-Donoso, R. and Page, J. and Berrios, S.}, year={2016}, month={Jun.} }