TY - JOUR AU - Huang, YH AU - Sun, MJ AU - Jiang, M AU - Fu, BY PY - 2009/08/17 Y2 - 2024/03/28 TI - Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells JF - European Journal of Histochemistry JA - Eur J Histochem VL - 53 IS - 2 SE - Original Papers DO - 10.4081/ejh.2009.e10 UR - https://www.ejh.it/ejh/article/view/ejh.2009.e10 SP - e10 AB - We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PPsecreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells. ER -