TY - JOUR AU - Bułdak, R.J. AU - Skonieczna, M. AU - Bułdak, Ł. AU - Matysiak, N. AU - Mielańczyk, Ł. AU - Wyrobiec, G. AU - Kukla, M. AU - Michalski, M. AU - Żwirska-Korczala, K. PY - 2014/09/12 Y2 - 2024/03/28 TI - Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment JF - European Journal of Histochemistry JA - Eur J Histochem VL - 58 IS - 3 SE - Original Papers DO - 10.4081/ejh.2014.2408 UR - https://www.ejh.it/ejh/article/view/2408 SP - AB - <p>The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.</p> ER -