TY - JOUR AU - Mitsuoka, Kazuyuki AU - Miwa, Yoko AU - Kikutani, Takeshi AU - Sato, Iwao PY - 2018/11/22 Y2 - 2024/03/29 TI - Localization of CGRP and VEGF mRNAs in the mouse superior cervical ganglion during pre- and postnatal development JF - European Journal of Histochemistry JA - Eur J Histochem VL - 62 IS - 4 SE - Original Papers DO - 10.4081/ejh.2018.2976 UR - https://www.ejh.it/ejh/article/view/2976 SP - AB - <p>The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14.5 (E14.5), E17.5 and postnatal day 1 (P1) using <em>in situ</em> hybridization. The antisense probe for CGRP was detected by <em>in situ</em> hybridization at E14.5, E17.5, and P1, and the highest levels were detected at E17.5. In contrast, the antisense probe for VEGF-A was detected by <em>in situ </em>hybridization in gradually increasing intensity from E14.5 to P1. The differences in the expression of these two markers revealed specific characteristics related to CGRP concentration and release compared to those of VEGF-A during development. The correlation between CGRP and VEGF-A may influence functional stress and the vascular blood supply during prenatal and postnatal development.</p> ER -