TY - JOUR AU - Le, Lulu AU - Luo, Ji AU - Wu, Haifang AU - Chen, Ling AU - Tang, Xiaoli AU - Fu, Fen PY - 2021/03/30 Y2 - 2024/03/29 TI - Overexpression of MYBL2 predicts poor prognosis and promotes oncogenesis in endometrial carcinoma JF - European Journal of Histochemistry JA - Eur J Histochem VL - 65 IS - 2 SE - Articles DO - 10.4081/ejh.2021.3226 UR - https://www.ejh.it/ejh/article/view/3226 SP - AB - <p>Endometrial cancer (EC) is the most common gynecologic malignancy and still remains clinically challenging. We aimed to explore the potential biomarkers of EC and provide a theoretical basis for early screening and targeted therapy. The available transcriptome data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were analyzed to identify differentially expressed genes. Immunohistochemistry was performed to detect gene expression. We analyzed the associations of <em>MYBL2</em> with clinicopathological features and survival time and the biological effect of <em>MYBL2</em> on the proliferation of EC cells. The effect of <em>MYBL2</em> silencing on the transcriptome of EC cell model was analyzed by RNA-Seq. <em>MYBL2</em> was significantly upregulated with obvious copy number alteration (CNA) in EC. Copy number amplification significantly increased <em>MYBL2</em> mRNA expression, which led to a poor prognosis and severe pathological types of EC. Additionally, <em>MYBL2</em> silencing significantly inhibited proliferation and induced apoptosis and G<sub>1</sub>-phase cell cycle arrest in EC cell lines. Our results indicate that <em>MYBL2</em> is closely related to the cell cycle and apoptosis pathways in EC. The findings in this study provide evidence that <em>MYBL2</em> can serve as a new candidate prognostic marker and a target for future therapeutic intervention in EC.</p> ER -