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Expression of the HPV18/E6 oncoprotein induces DNA damage

Elva I. Cortés Gutiérrez, Catalina García-Vielma, Adriana Aguilar-Lemarroy, Veronica Vallejo-Ruíz, Patricia Piña-Sánchez, Pablo Zapata-Benavides, Jaime Gosalvez
  • Catalina García-Vielma
    Instituto Mexicano del Seguro Social, Mexico
  • Adriana Aguilar-Lemarroy
    Instituto Mexicano del Seguro Social, Mexico
  • Veronica Vallejo-Ruíz
    Instituto Mexicano del Seguro Social, Mexico
  • Patricia Piña-Sánchez
    Instituto Mexicano del Seguro Social, Mexico
  • Pablo Zapata-Benavides
    Instituto Mexicano del Seguro Social, Mexico
  • Jaime Gosalvez
    Universidad Autónoma de Madrid, Spain

Abstract

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection–fluorescence in situ hybridization (DBD–FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.

Keywords

DNA damage; DNA breakage detection-fluorescence in situ hybridization; DBD-FISH; human papillomavirus; HPV; E6 oncogene; alkaline-labile sites.

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Submitted: 2017-01-17 02:23:41
Published: 2017-06-15 13:42:21
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Copyright (c) 2017 Elva I. Cortés Gutiérrez, Catalina García-Vielma, Adriana Aguilar- Lemarroy, Veronica Vallejo-Ruíz, Patricia Piña-Sánchez, Pablo Zapata-Benavides, Jaime Gosalvez

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