Cover Image

Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura
  • Yu-Ki Ohmori
    Dental School Okayama University, Japan
  • Mai Nishino
    Dental School Okayama University, Japan
  • Masayo Yoshida
    Dental School Okayama University, Japan
  • Kaori Tabata
    Dental School Okayama University, Japan
  • Do-Saku Hirota
    Dental School Okayama University, Japan
  • Ayako Ryu-Mon
    Dental School Okayama University, Japan
  • Hiromitsu Yamamoto
    Kyoto University, Japan
  • Junya Sonobe
    Kyoto University, Japan
  • Yo-Hei Kataoka
    Okayama University, Japan
  • Noriko Shiotsu
    Okayama University, Japan
  • Mika Ikegame
    Okayama University, Japan
  • Hiroki Maruyama
    Niigata University, Japan
  • Toshio Yamamoto
    Okayama University, Japan
  • Kazuhisa Bessho
    Kyoto University, Japan
  • Kiyoshi Ohura
    Osaka Dental University, Japan

Abstract

We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regeneration-related markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.

Keywords

Cell fate; skeletal muscle; BMP; gene transfer; in vivo electroporation.

Full Text

PDF
Submitted: 2017-01-13 08:22:38
Published: 2017-05-05 15:52:52
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
330

Views:
PDF
53

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2017 Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185