Cover Image

Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii)

Liqin Xi, Chen Wang, Pengyu Chen, Qi Yang, Ruiqi Hu, Haolin Zhang, Qiang Weng, Meiyu Xu
  • Liqin Xi
    Beijing Forestry University, China
  • Chen Wang
    Beijing Forestry University, China
  • Pengyu Chen
    Beijing Forestry University, China
  • Qi Yang
    Beijing Forestry University, China
  • Ruiqi Hu
    Beijing Forestry University, China
  • Haolin Zhang
    Beijing Forestry University, China
  • Qiang Weng
    Beijing Forestry University, China

Abstract

The cytokine interleukin-6 (IL-6) mediates a wide range of inflammatory and immune responses. Tumor Necrosis Factor α (TNF-α) has a myriad of pro-inflammatory effects on the skin. In this study, we investigated the localizations and expression levels of IL-6, TNF-α and NF-κB in the skin of Rana dybowskii during the breeding period and pre-hibernation. Histologically, the skin of Rana dybowskii consists of epidermis and dermis. Four kinds of cells were identified in the epidermis during the breeding period and pre-hibernation, while the dermis was composed of homogenous gel, mucous glands and granular glands. IL-6, TNF-α and NF-κB were immunolocalized in the epithelial and glandular cells in both periods. Western blotting showed that IL-6, TNF-α and NF-κB were significantly higher in the pre-hibernation compared to the breeding period. Real-Time PCR revealed that the relative mRNA levels of IL-6 and NB-κB in the pre-hibernation increased significantly compared with the breeding period, while the TNF-α mRNA expression levels were no significant difference between these two periods. These results suggested that IL-6, TNF-α and NF-κB may collectively play important roles in the skin immune system of Rana dybowskii during the breeding period and pre-hibernation.

Keywords

IL-6; TNF-α; NF-кB; Rana dybowskii; skin

Full Text

PDF
Submitted: 2017-07-16 07:17:55
Published: 2017-10-19 09:47:26
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
106

Views:
PDF
47

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2017 Liqin Xi, Chen Wang, Pengyu Chen, Qi Yang, Ruiqi Hu, Haolin Zhang, Qiang Weng, Meiyu Xu

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185