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The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin

Michelle Steicke, Guang Yang, Tam Nguyen Dinh, Matthew Dunster-Jones, Owen Sargisson, Farah Ahmady, Jonathan Golledge, Yutang Wang
  • Michelle Steicke
    Federation University Australia, School of Applied and Biomedical Science, Australia
  • Guang Yang
    Department of Geriatric Cardiology, Qianfoshan Hospital Affiliated to Shandong University, China | yangg1972@126.com
  • Tam Nguyen Dinh
    Federation University Australia, School of Applied and Biomedical Science, Australia
  • Matthew Dunster-Jones
    Federation University Australia, School of Applied and Biomedical Science, Australia
  • Owen Sargisson
    Federation University Australia, School of Applied and Biomedical Science, Australia
  • Farah Ahmady
    Federation University Australia, School of Applied and Biomedical Science, Australia
  • Jonathan Golledge
    James Cook University, College of Medicine and Dentistry The Townsville Hospital, Department of Vascular and Endovascular Surgery, Australia
  • Yutang Wang
    Federation University Australia, School of Applied and Biomedical Science, Australia

Abstract

Methanol, ethanol and formalin are commonly used as fixatives to preserve biological tissues from decay in the preparation of histological sections. Fixation of the inner layers of the tissue depends on the ability of the fixative to diffuse into the tissue. It is unknown whether methanol penetrates tissues at similar rates to other fixatives. This study aimed to compare the penetration rates of methanol, ethanol and formalin into bovine heart and liver tissues. The penetration distance and tissue shrinkage or expansion were measured by analysing the digital images of tissue before and after immersion in different fixatives for 1, 2, 6 or 10 h. Data were analysed using two-way ANOVA, followed by Bonferroni’s post-hoc test. The penetration distance of methanol was significantly greater in both heart and liver tissues compared with that of ethanol (N=4, P<0.001). Methanol or ethanol immersion led to similar shrinkage of both tissues (P>0.05). The penetration rate of formalin was similar to that of ethanol in both tissues however it was significantly slower than methanol (N=4, P<0.005 in the heart; P<0.001 in the liver). The mean penetration coefficients of methanol, formalin and ethanol in the heart tissue were 2.609, 1.994 and 1.801, respectively, and 3.012, 2.153 and 2.113, respectively, in the liver tissue. The penetration coefficient of methanol was significantly greater than that of ethanol or formalin in both tissues (P<0.001 for each comparison). In conclusion, methanol penetrates tissue significantly faster than ethanol and formalin.

Keywords

Ethanol; formalin; methanol; penetration coefficient; penetration rate.

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Submitted: 2017-12-08 08:29:59
Published: 2018-02-07 10:04:08
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Copyright (c) 2018 Michelle Steicke, Guang Yang, Tam Nguyen Dinh, Matthew Dunster-Jones, Owen Sargisson, Farah Ahmady, Jonathan Golledge, Yutang Wang

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