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Histochemical and morphological aspects of fresh frozen bone: a preliminary study

F.S. De Ponte, G. Cutroneo, R. Falzea, G. Rizzo, L. Catalfamo, A. Favaloro, G. Vermiglio, M. Runci, A. Centofanti, G. Anastasi
  • F.S. De Ponte
    University of Messina, Italy
  • G. Cutroneo
    University of Messina, Italy
  • R. Falzea
    University of Messina, Italy
  • G. Rizzo
    University of Messina, Italy
  • L. Catalfamo
    University of Messina, Italy
  • A. Favaloro
    University of Messina, Italy
  • M. Runci
    University of Messina, Italy
  • A. Centofanti
    University of Messina, Italy
  • G. Anastasi
    University of Messina, Italy

Abstract

Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.

Keywords

Fresh frozen bone; osteoclast; osteoblast; VEGF; fluorescence antibody technique; histological techniques.

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Submitted: 2016-03-16 13:57:19
Published: 2016-12-06 15:29:21
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Copyright (c) 2016 Francesco Saverio De Ponte, Giuseppina Cutroneo, Roberto Falzea, Giuseppina Rizzo, Luciano Catalfamo, Angelo Favaloro, Giovanna Vermiglio, Michele Runci, Antonio Centofanti, Giuseppe Anastasi

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