Can a genetically-modified organism-containing diet influence embryo development? A preliminary study on pre-implantation mouse embryos

B Cisterna, F Flach, L Vecchio, SML Barabino, S Battistelli, TE Martin, M Malatesta, M Biggiogera
  • F Flach
    Affiliation not present
  • L Vecchio
    Affiliation not present
  • SML Barabino
    Affiliation not present
  • S Battistelli
    Affiliation not present
  • TE Martin
    Affiliation not present
  • M Malatesta
    Affiliation not present
  • M Biggiogera
    Affiliation not present

Abstract

In eukaryotic cells, pre-mRNAs undergo several transformation steps to generate mature mRNAs. Recent studies have demonstrated that a diet containing a genetically modified (GM) soybean can induce modifications of nuclear constituents involved in RNA processing in some tissues of young, adult and old mice. On this basis, we have investigated the ultrastructural and immunocytochemical features of pre-implantation embryos from mice fed either GM or non- GM soybean in order to verify whether the parental diet can affect the morpho-functional development of the embryonic ribonucleoprotein structural constituents involved in premRNA pathways. Morphological observations revealed that the general aspect of embryo nuclear components is similar in the two experimental groups. However, immunocytochemical and in situ hybridization results suggest a temporary decrease of pre-mRNA transcription and splicing in 2-cell embryos and a resumption in 4-8-cell embryos from mice fed GM soybean; moreover, pre-mRNA maturation seems to be less efficient in both 2-cell and 4-8-cell embryos from GM-fed mice than in controls. Although our results are still preliminary and limited to the pre-implantation phases, the results of this study encourage deepening on the effects of food components and/or contaminants on embryo development.

Full Text

PDF
Submitted: 2009-08-17 09:35:23
Published: 2009-08-17 09:40:53
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
220

Views:
PDF
283

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2009 B Cisterna, F Flach, L Vecchio, SML Barabino, S Battistelli, TE Martin, M Malatesta, M Biggiogera

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185