17th International Conference of Histochemistry and Cytochemistry, 2025

All Items

• IMMUNOLOGICAL MECHANISMS IN REGENERATIVE MEDICINE: A LESSON FROM THE HUMAN PLACENTA O. Parolini^1,2 | ¹Department of Life Science and Public Health, Università Cattolica del Sacro Cuore, Rome, Italy; ²Fondazione IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy

  https://doi.org/10.4081/ejh.2025.4266

  343

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• P85 | ALTERATION OF SUBMUCOSAL AND MYENTERIC GANGLIA IN SEVERE GUT DYSMOTILITY: A QUANTITATIVE MORPHOMETRIC ANALYSIS H. Yaghi¹, E. Boschetti¹, S. Blando¹, I. Neri¹, C. Malagelada², R. De Giorgio³, L. Manzoli¹, S. Ratti¹ | ¹Center of Clinical Surgical Molecular and Experimental Anatomy Alma Mater Studiorum, University of Bologna, Bologna, Italy; ²Departament de Medicina, Universitat Autonoma de Barcelona, Barcelona, Spain; ³Department of Translational Medicine, Università di Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4411

  260

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• P53 | CIRCADIAN DEPENDENT MOTILITY: THE ROLE FOR THE PERINUCLEAR ACTIN CAP E. Montacci¹, M. Sgarzi², L. Dall’Olio³, E. Giampieri⁴, D. Romaniello⁴, M. Lauriola⁵ | ¹Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; ²IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ³Laboratory of Data Science and Bioinformatics, IRCSS Institute of Neurological Sciences, Bellaria Hospital, Bologna, Italy; ⁴Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; ⁵Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4375

  271

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• P20 | RNASEQ AND HISTOCHEMICAL STAININGS ASSESS BIOCHEMICAL AND STRUCTURAL CHANGES IN THE MOUSE INTERVERTEBRAL DISC ACROSS THE LIFESPAN A. Hallmark¹, A. Ali², C. Dahia³ | ¹Hospital for Special Surgery, New York, USA; ²Department of Medicine, Columbia University, New York, USA; ³Hospital for Special Surgery, Weill Cornell Medicine

  https://doi.org/10.4081/ejh.2025.4340

  171

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• TRACKING HER2 TRAFFICKING AND ORGANELLE CROSSTALK IN TRASTUZUMAB-DERUXTECAN–TREATED BREAST CANCER CELLS USING MULTIMODAL IMAGING E. Tagliatti¹, M. C. Gagliani², G. Bellese², M. Crippa³, A. Abbona⁴, M. Paccagnella⁴, P. Arnaldi⁵, M. C. Merlano⁶, O. Garrone⁷, A.M. Porcelli⁸, M. Matteoli¹, P. Falletta⁹, P. Castagnola¹⁰, K. Cortese² | ¹IRCCS Humanitas Research Hospital, Laboratory of Pharmacology and Brain Pathology, Rozzano, Milano, Italy; ²DIMES, Department of Experimental Medicine, Cellular Electron Microscopy Lab, University of Genoa, Genoa, Italy; ³Experimental Imaging Center, IRCCS Ospedale San Raffaele, Milan, Italy; ⁴Health Department S. Croce e Carle Teaching Hospital, Cuneo, Italy; ⁵IRCCS Ospedale Policlinico San Martino, Genova, Italy; ⁶Scientific Direction, Candiolo Cancer Institute, FPO-IRCCS, Candiolo, Italy; ⁷Medical Oncology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano, Italy; ⁸FABIT, Department of Pharmacy and Biotechnology and CIRI, Interdepartmental Centre for Industrial Research ‘Scienze Della Vita e Tecnologie per La Salute’, University of Bologna, Bologna, Italy; ⁹Vita-Salute San Raffaele University, Milan, Italy; ¹⁰IRCCS Ospedale Policlinico San Martino, Genova, Italy

  https://doi.org/10.4081/ejh.2025.4305

  304

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• A TECHNOLOGY PLATFORM FOR TRANSLATION OF ATMPS (ADVANCED THERAPY MEDICINAL PRODUCTS): THE EXAMPLE OF HUMAN EYE AND SKIN G. Pellegrini | Head of Cell Therapy Program Center for Regenerative Medicine Department of Life Sciences University of Modena e Reggio Emilia, Modena, Italy

  https://doi.org/10.4081/ejh.2025.4269

  294

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• P86 | THE EFFECTS OF NICOTINIC ACETYLCHOLINE RECEPTOR INHIBITION ON THE EXPRESSION OF BRAIN DERIVED NEUROTROPHIC FACTOR IN THE OLFACTORY BULB ORGANOTYPIC SLICE CULTURES I. Atasoy¹, S. Yilmazer², D. Gezen Ak³, E. Dursun³ | ¹Medical Biology Department, İstabul University-Cerrahpasa Faculty of Medicine Istanbul, Türkiye; ²Department of Medical Biology Halic University, Faculty of Medicine, Istanbul, Türkiye; ³Brain and Neurodegenerative Disorders Research Laboratories, Department of Neuroscience, Institute of Neurological Sciences, Istanbul University-Cerrahpasa, Istanbul, Türkiye

  https://doi.org/10.4081/ejh.2025.4412

  276

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• PO54 | FATTY INFILTRATION AND POTENTIAL ADIPOGENIC SHIFT OF SKELETAL MUSCLE CELLS: MULTIPARAMETRIC HISTOLOGICAL ANALYSIS OF MYOSTEATOSIS L. Morello¹, G. Stati¹, V. Giansante¹, S. Lattanzio¹, P. Cerritelli¹, D. Bruni¹, S. Cinti², R. Di Pietro^1,3,4 | ¹Department of Medicine and Aging Sciences, “G. d’ Annunzio” University of Chieti-Pescara, Chieti, Italy; ²Department of Experimental and Clinical Medicine, Center of Obesity, Polytechnic University of Marche, Ancona, Italy; ³StemTeCh Group, G. d’ Annunzio Foundation, “G. d’ Annunzio” University of Chieti Pescara, Chieti, Italy; ⁴Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA, USA

  https://doi.org/10.4081/ejh.2025.4379

  291

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• P21 | EARLY BIOLOGICAL EFFECTS ON CELL PROLIFERATION AND DIFFERENTIATION INDUCED BY EXTRACELLULAR MICROVESICLES (EVS) DERIVED FROM HUMAN KERATINOCYTES IN PSORIATIC MICROENVIRONMENT D. Daluiso¹, B. Barco¹, C. Porro², E. Donetti³, F. Riva¹ | ¹Department of Publich Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy; ²Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy; ³Department of Biomedical Sciences for Health, University of Milan, Milan, Italy

  https://doi.org/10.4081/ejh.2025.4341

  175

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• SPATIO-TEMPORAL ORGANIZATION OF THE SUPER-ENHANCER STRUCTURE IN CANCER CELLS A. Chytła¹, E. H. Lagunar¹, R. Gonül¹, B. Šalovská², J. Červenka³, A. Miladinović⁴, L. Antiga⁴, P. Hozák⁴, M. Sztacho¹ | ¹Laboratory of Cancer Cell Architecture, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic; ²Yale Cancer Biology Institute, Yale University School of Medicine, West Haven, USA; ³Laboratory of proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic; ⁴Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic

  https://doi.org/10.4081/ejh.2025.4306

  272

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• ANTIBODY-TO-IMAGING PIPELINE TO MONITOR DRUG-TARGET ENGAGEMENT IN BREAST TUMORS M. Barroso | Department of Molecular and Cellular Physiology Albany Medical College, Albany NY 12208, USA

  https://doi.org/10.4081/ejh.2025.4270

  297

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• P87 | EVALUATION OF SPEXIN AND KISSPEPTIN EXPRESSIONS IN ADIPOSE TISSUES OF WISTAR ALBINO RATS DURING PUBERTY A.H. Yozgat¹, D. Billur¹, B.I Erguder², A. Ucar³ | ¹Ankara University Faculty of Medicine, Department of Histology and Embryology, Ankara, Türkiye; ²Ankara University Faculty of Medicine, Department of Medical Biochemistry, Ankara, Türkiye; ³University of Health Sciences, Sisli Hamidiye Etfal Health Practices and Research Centre, Department of Pediatric Endocrinology and Diabetes, Istanbul, Türkiye

  https://doi.org/10.4081/ejh.2025.4413

  269

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• P55 | ENDOTHELIAL THYMIDINE PHOSPHORYLASE DEFICIENCY TRIGGERS MORPHO-FUNCTIONAL DEGE-NERATION OF ENTERIC NEURONS AND REDUCES INTESTINAL STEM CELL PROLIFERATION I. Neri¹, E. Boschetti¹, T. Saha², S. Rommel², S. Schulte², M. Gries², R. De Giorgio³, L. Manzoli¹, S. Ratti¹, K. Schäfer² | ¹Cellular Signalling Laboratory, Anatomy Center, Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy; ²Workgroup Enteric Nervous System (AGENS), Department of Informatics and Microsystems Technology, University of Applied, Sciences Kaiserslautern, Zweibrücken, Germany; ³Department of Translational Medicine, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4380

  265

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• P22 | EXPLORING THE UNEXPECTED DUAL ROLE OF GARLIC DERIVATIVES IN REGULATING INVASIVENESS IN BREAST CANCER SUBTYPES M. Dell’Aira¹, S. Grassilli², M. Pierantoni¹, V. Bertagnolo¹, F. Brugnoli¹ | ¹Department of Translational Medicine, University of Ferrara, Ferrara, Italy; ²Department of Environmental Sciences and Prevention and LTTA Centre, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4342

  224

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• ADVANCEMENTS AND EMERGING PERSPECTIVES IN MICROSCOPIC IMAGING S. Karahuseyinoglu | Department of Histology & Embryology, Koc University, School of Medicine, Istanbul, Turkiye

  https://doi.org/10.4081/ejh.2025.4307

  250

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• HUMAN AMNIOTIC MEMBRANE POTENTIAL FOR BONE REPAIR: FROM THE LAB TO THE CLINIC - ROLE OF ITS IMMUNOGENICITY F. Gindraux^1,2 | ¹CHU Besançon, Service de Chirurgie Maxillo-Faciale et Stomatologie, Besançon, France; ²Université Marie et Louis Pasteur, SINERGIES UR 4662, Besançon, France

  https://doi.org/10.4081/ejh.2025.4271

  177

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• P88 | REGENERATIVE MEDICINE, REHABILITATION AND CYTO-HISTOCHEMISTRY: RECENT EVALUATIONS OF INTEGRATED TREATMENTS L. Gatta^1,2, M. Ceccarelli^1,3, C. Del Gaizo¹ | ¹BIORI, Academy of Bioregenerative Medicine and Surgery; Rome, Italy; ²Catholic University of Rome, Policlinico Gemelli, Rome, Italy; ³International Center for Study and Research in Physiological and Aesthetic Medicine (Ae. Phy. Med. Centre), Rome, Italy.

  https://doi.org/10.4081/ejh.2025.4414

  255

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• P56 | HISTOLOGICAL PROFILE OF INACTIVE UNILATERAL CONDYLAR HYPERPLASIA: EVIDENCE OF BORDERLINE TISSUE ACTIVITY F. Nicita¹, G. Vermiglio¹, J. Freni¹, A. Centofanti¹, D. Labellarte¹, R. Briganti¹, G.P. Anastasi¹, G. Cutroneo¹, A. Favaloro¹, M. Runci Anastasi² | ¹Department of Biomedical, Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy; ²Department of Maxillo-Facial Surgery, University of Rome, La Sapienza, Italy

  https://doi.org/10.4081/ejh.2025.4381

  278

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• P23 | CALRETININ IMMUNOREACTIVE NON-TRADITIONAL LARGE NEURONS IN THE GRANULAR LAYER OF THE HUMAN CEREBELLAR CORTEX P. Flace¹, M. E. Caringella², D. Galletta³, G. Santangelo³, C. Caporusso², M. Stolfa², A. Cacciola⁴, J.J.V. Branca⁵, G. Agazzino², R. Gradini⁶, P. Livrea⁷, A. Marzullo² | ¹Medical School, University of Bari ‘Aldo Moro’, Bari, Italy; ²Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari ‘Aldo Moro’, Italy; ³Department of Psychology, Specialization School of Neuropsychology. University of Campania ‘Luigi Vanvitelli’, Caserta, Italy; ⁴Brain Mapping Lab, Department of Biomedical, Dental Sciences and Morphological and Functional Imaging, University of Messina, Messina, Italy; ⁵Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italy; ⁶Department of Experimental Medicine, Sapienza University, Roma, Italy; ⁷University of Bari ‘Aldo Moro’, Bari, Italy

  https://doi.org/10.4081/ejh.2025.4343

  158

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• IMAGING THE LYSOSOME IN CELLULAR SYSTEMS FOR DRUG DISCOVERY AND SCREENING V.A. Baldassarro¹, M. Galeotti², E. Satanassi³, L. Giardino¹, L. Calzà⁴, C. Quadalti⁴ | ¹DIMEVET, University of Bologna, Italy; ²IRET Foundation, Ozzano dell’Emilia, Bologna, Italy; ³Interdepartmental Centre for Industrial Research in Health Sciences and Technology ICIR-HST, University of Bologna, Ozzano dell’Emilia, Bologna, Italy; ⁴Fabit, University of Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4308

  292

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• HUMAN AMNIOTIC EPITHELIAL CELLS FOR RETINAL REGENERATION: INVESTIGATING THEIR DIFFERENTIATIVE POTENTIAL IN VITRO R. Pisu¹, S. Saponara², S. Angioni², E. Peiretti², S.G. Vitale², F. Marongiu¹ | ¹Department of Biomedical Sciences, University of Cagliari, Cagliari; ²Department of Surgical Sciences, University of Cagliari, Cagliari, Italy

  https://doi.org/10.4081/ejh.2025.4272

  193

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• P57 | SELONSERTIB (ASK1 INHIBITOR) IN SKIN TISSUE REPAIR OF OBESE MICE V. Oliveira Collares Barbos, B. Romana de Souza | Department of Histology and Embriology, State University of Rio de Janeiro, Rio de Janeiro, Brazil

  https://doi.org/10.4081/ejh.2025.4382

  272

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• P24 | DEVELOPMENT OF HEAD AND NECK SQUAMOUS CELL CARCINOMA PATIENT-DERIVED XENOGRAFTS (PDXS) AND ORGANOIDS (PDXOS) FOR MORPHOLOGICAL ANALYSIS AND DRUG SCREENING A. Francia¹, C. Girone¹, D.M. Filippini², G. Querzoli³, M. Fermi⁴, S. Venturoli⁵, A. Degiovanni⁶, C. Miroglio¹, E. Montacci¹, A. Ardizzoni⁷, M. Lauriola⁸, D. Romaniello⁸ | ¹Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; ²Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Division of Medical Oncology, IRCCS; Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ³Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Pathology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ⁴Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Department of Otorhinolaryngology-Head and Neck Surgery, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ⁵Microbiology Unit, IRCCS Azienda Ospedaliero-Universitaria of Bologna, Bologna, Italy; ⁶Pathology Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna,Bologna, Italy; ⁷Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Division of Medical Oncology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ⁸Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4344

  191

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• ASTROCYTE DYSFUNCTION IN AUTOSOMAL DOMINANT LEUKODYSTROPHY (ADLD): INSIGHTS FROM 2D AND 3D IN VITRO SYSTEMS AND EX VIVO CEREBROSPINAL FLUID METABOLOMICS F.D. Koufi¹, V. Righi², A. Mucci³, I. Rusciano¹, S. Mongiorgi¹, M. Shin⁴, Y. Kosodo⁴, I. Cani⁵, P. Cortelli⁶, E. Giorgio⁷, G. Ramazzotti¹, L. Manzoli¹, S. Ratti¹ | ¹ Cellular Signaling Laboratory, Anatomy Center, Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy; ²Department of Life Quality Studies, University of Bologna, Campus of Rimini, Rimini, Italy; ³Department of Geological and Chemical Sciences, University of Modena and Reggio Emilia, Modena, Italy; ⁴Korea Brain Research Institute (KBRI), Daegu, Republic of Korea; ⁵Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy; ⁶IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy; ⁷Department of Molecular Medicine, University of Pavia, Pavia, Italy; Medical Genetics Unit, IRCCS Mondino Foundation, Pavia, Italy.

  https://doi.org/10.4081/ejh.2025.4312

  314

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• PERICYTE INVOLVEMENT IN THE PATHOGENESIS OF COLVI-RELATED MYOPATHIES: INSIGHTS INTO NG2–COLVI AXIS A. Fazio¹, C. Evangelisti¹, P. Sabatelli², A. Cappellini¹, A. Di Martino³, C. Faldini³, L. Manzoli¹, S. Ratti¹ | ¹Cellular Signalling Laboratory, University of Bologna, DIBINEM, Bologna, Italy; ²CNR Institute of Molecular Genetics «Luigi Luca Cavalli-Sforza”, Unit of Bologna, Bologna, Italy; ³IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4273

  158

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• P58 | VITAMIN D ATTENUATES LIVER INJURY BY REDUCING APOTOSIS, PYROPTOSIS, FERROPTOSIS AND PROMOTING REGENERATION IN TYPE 2 DIABETES S. Rzeyeva¹, N. Bayromava², T. Ozbilenler¹, A. Akdemir³, M. Anapalı Aykaç⁴, D. Aydemir⁵, F. Kaya Dagistanli¹, T. Ulutin¹, O. Uysal¹, M. Ozturk⁶ | ¹Istanbul University-Cerrahpasa, Faculty of Medicine, Istanbul, Türkiye; ²Istanbul University-Cerrahpasa, Faculty of Medicine, Istanbul, Türkiye; ³Karabuk University, Faculty of Medicine, Karabük, Türkiye; ⁴Ataturk University, Faculty of Medicine, Erzurum, Türkiye; ⁵Istanbul University, Faculty of Medicine, Istanbul, Türkiye; ⁶Istanbul University-Cerrahpasa, Faculty of Medicine, Istanbul Türkiye

  https://doi.org/10.4081/ejh.2025.4383

  252

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• P25 | RESPONSE TO HEDGEHOG AND PI3K/AKT/MTOR PATHWAY INHIBITORS IN PREB-ALL: ANALYSIS OF THE INTRACELLULAR DISTRIBUTION OF PATHWAY ENZYMES AND OF THE AUTOPHAGY PROCESS M. Sicurella¹, M. De Chiara², E. Battaglia², F. Franco², M. De Vita², L.M. Neri² | ¹Department of Environmental and Prevention Sciences, University of Ferrara, Ferrara, Italy; ²Department of Translational Medicine, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4345

  195

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• THE FACETED S100B PROTEIN: THE JEKYLL/HYDE SIDE F. Michetti | LUM University Casamassima, Genes Company, Roma, Italy

  https://doi.org/10.4081/ejh.2025.4313

  271

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• THE AUTOPHAGY-INDEPENDENT ROLE OF ATG7 IN REGULATING NEUROGENESIS Y. Shen, G. Wang | International Joint Laboratory for Embryonic Development & Prenatal Medicine, Division of Histology and Embryology, Medical College, Jinan University, Guangzhou, China

  https://doi.org/10.4081/ejh.2025.4274

  207

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• P59 | IMMUNOHISTOCHEMISTRY ANALYSIS TO DETECT A MOLECULAR SIGNATURE IN INTERVERTEBRAL DISC DEGENERATION L. Penolazzi¹, R. Nadalini¹, C. Sief¹, E. Lambertini², C. Angelini³, R. Piva¹ | ¹Department of Neurosciences and rehabilitation, University of Ferrara, Italy; ²Laboratorio Centralizzato di ricerca preclinica, University of Ferrara, Italy; ³Department of Translational Medicine, University of Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4384

  268

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• P26 | HUMAN CYTOMEGALOVIRUS-DRIVEN ADAPTIVE NK CELLS AND MATRIX METALLOPROTEINASES EXPRESSION AS KEY ELEMENTS IN ATHEROSCLEROTIC PLAQUE DESTABILIZATION J. Freni¹, M. Crescenti², E. Bertuccio³, A. Fittipaldi¹, P. Carrega², E. Di Carlo⁴, G. Ferlazzo³, G. Vermiglio¹, I. Bonaccorsi² | ¹Department of Biomedical, Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy: ²Department Human Pathology, «G. Barresi», University of Messina, Messina, Italy; ³Department of Experimental Medicine (DIMES), University of Genoa, Genova, Italy; ⁴Department of Medicine and Sciences of Aging, «G. D’Annunzio» University of Chieti-Pescara, Chieti, Italy

  https://doi.org/10.4081/ejh.2025.4346

  197

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• HOST/PATHOGEN INTERACTIONS AND THE ROLE OF THE BARRIERS: ON THE EDGE BETWEEN HEALTH AND MULTIFACTORIAL/COMPLEX DISEASES G. Di Sante¹, A. M. Stabile¹, A. Pistilli¹, M. Ruggirello^1,2, F. Michetti^3,4, F. Ria², M. Rende¹ | ¹ Department of Surgery and Medicine, Sect. of Human, Clinical and Forensic Anatomy, University of Perugia, Perugia, Italy; ²Department of Translational Medicine and Surgery, Section of General Pathology, University “Cattolica del Sacro Cuore” of Rome, Roma, Italy; ³Department of Medicine, LUM University, Casamassima, Bari, Italy; ⁴Italy Genes, Rome, Italy

  https://doi.org/10.4081/ejh.2025.4314

  269

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• UNRAVELING THE RELATIONSHIPS BETWEEN TUMOR CELLS AND THEIR MICROENVIRONMENT USING HIGH-RESOLUTION SPATIAL TRANSCRIPTOMICS M. Cordenonsi |Department of Molecular Medicine, University of Padova, Padova, Italy

  https://doi.org/10.4081/ejh.2025.4275

  165

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• P60 | UNDERSTANDING HUMAN INTERVERTEBRAL DISC HOMEOSTASIS: 3D EXPERIMENTAL MODELS L. Penolazzi¹, A. Chierici¹, R. Nadalini¹, E. Lambertini², C. Nastruzzi³, G. Lisignoli⁴, C. Manferdini⁴, G. D’Atri⁴, R. Piva¹ | ¹Department of Neurosciences and rehabilitation, University of Ferrara, Italy; ²Laboratorio centralizzato di ricerca preclinica, University of Ferrara, Italy; ³Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Italy; ⁴Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4385

  262

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• P27 | HISTOCHEMICAL APPROACHES FOR EVALUATING THE REPRODUCTIVE HEALTH OF MUSSEL MYTILUS GALLOPROVINCIALIS EXPOSED TO ACUTE THERMAL STRESS M. Galati, M. A. Oueldi, M. Romeo, T. Cappello, M. Maisan | Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy

  https://doi.org/10.4081/ejh.2025.4347

  183

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• ON THE WAY TO A NEW MODEL OF NEURODEGENERATION? FIRST EVIDENCE OF THE EXPRESSION OF GATA1 IN THE BRAIN: CORRELATIONS WITH AGING, MITOCHONDRIAL DAMAGE AND SYNUCLEINOPATHY C. Caturano¹, F. E. Bellomi¹, F. Arciprete¹, V. Velardi¹, L. La Barbera², M. Falchi³, M. Bacioglu⁴, R. Mancinelli⁵, M. D’Amelio², M. Spillantini⁴, M. Zingariello¹, G. Vivacqua^1,6 | ¹Laboratory of Microscopic and Ultrastructural Anatomy, Campus Biomedico University of Rome, Italy; ²Laboratory of Molecular Neuroscience - Campus Biomedico University of Rome, Italy; ³National Centre for HIV/AIDS Research, Istituto Superior di Sanità, Rome, Italy; ⁴Department of Clinical Neuroscience, The University of Cambridge, Cambridge, UK; ⁵Department of Anatomic, Histologic, Forensic Medicine and Locomotor Apparatus Science, Sapienza University of Rome, Italy; ⁶Department of Clinical Neuroscience, The University of Cambridge, Cambridge, UK

  https://doi.org/10.4081/ejh.2025.4315

  274

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• ROLE OF SCHWANN CELLS-CANCER CELLS CROSSTALK IN TUMOUR PROGRESSION C. Giampietri¹, E. Pizzichini¹, F. Somma¹, S. Petrungaro¹, A. Facchiano², A. Filippini¹, C. Fabrizi¹, E. Gaudio¹ | ¹Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Sapienza University of Rome, Rome, Italy; ²Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Rome, Italy

  https://doi.org/10.4081/ejh.2025.4276

  189

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• P61 | IMMUNOPROFILE OF MERKEL CELLS IN THE HUMAN VAGINAL EPITHELIUM S. Polakovičová¹, M. Borovský², L. Maršík², B. Filová³, I. Varga³, L. Sallicandro⁴ | ¹Inst. of Hist. and Embr. MFCU in Bratislava, Bratislava, Slovakia; ²1st Obst. and Gyn. Dep. FMCU with Repr. Med. Centre Hospital, Bratislava, Slovakia; ³Inst. of Hist. and Embr. MFCU in Bratislava, Bratislava, Slovakia; ⁴Dep. of Medicine and Surgery, University of Perugia, Perugia, Italy

  https://doi.org/10.4081/ejh.2025.4386

  240

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• P28 | EXPLORING THE POTENTIAL LINK BETWEEN MITOCHONDRIAL DYSFUNCTION AND MITO-EVS IN PANCREATIC DUCTAL ADENOCARCINOMA (PDAC) V. Giansante¹, L. Leanza², E. Guerra³, F. Vianello⁴, C. Iacovino¹, S. Lattanzio¹, L. Morello¹, G. Stati¹, P. Simeone³, P. Lanuti³, S. Alberti⁵, M. Reichert⁶, R. Di Pietro¹ | ¹Department of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; ²Department of Biology, University of Padua, Padua, Italy; ³Department of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Laboratory of Cancer Pathology, Center for Advanced Studies and Technology (CAST)G. d’Annunzio” University of Chieti-Pescara, Italy; ⁴Department of Biology, University of Padua, Padua, Italy; ⁵Unit of Medical Genetics, Department of Biomedical Sciences, University of Messina, Messina, Italy; ⁶Translational Pancreatic Cancer Research Center, Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany; Center for Organoid Systems (COS), Technical University of Munich, Garching, Germany

  https://doi.org/10.4081/ejh.2025.4348

  192

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• IMPAIRMENT OF HERP E3 LIGASE ACTIVITY BY MUTANT POLYGLUTAMINE PROTEINS: A NOVEL MECHANISM IN POLYGLUTAMINE DISEASE NEUROPATHOLOGY T. Peng, B. Zhang, M. Yang, H. Li | Department of Histology and Embryology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

  https://doi.org/10.4081/ejh.2025.4316

  264

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• CAPTURE-CAPIVASERTIB AND PROSTATE CANCER TARGETING: UNRAVELING RESISTANCE ELEMENTS M. Zavatti¹, V. Serafin¹, L. Antolini¹, S. Bresolin², L. Reggiani Bonetti³, S. Marmiroli¹ | ¹Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; ²Pediatric Hematology, Oncology and Stem Cell Transplant Division, Women and Child Health Department, Padua University, Padua, Italy; ³Department of Medical and Surgical Sciences. University of Modena and Reggio Emilia, Modena, Italy

  https://doi.org/10.4081/ejh.2025.4277

  198

---

• P62 | FFAR4/GPR120 ACTIVATION COUNTERACTS FIBROGENIC PATHWAYS IN A CHOLESTATIC LIVER INJURY MODEL S. Pompili¹, R. Sferra¹, F. Ronca¹, A. Cappariello², A. Vetuschi¹ | ¹Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, L’Aquila, Italy; ²Department of Life, Health and Environmental Sciences, University of L’Aquila, L’Aquila, Italy

  https://doi.org/10.4081/ejh.2025.4387

  274

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• P29 | GLUTAMATERGIC MODULATION OF HYPOTHALAMIC RSPONDIN3 NEURONS UNDER PHYSIOLOGICAL CONDITIONS D. Gok Yurtseven¹, G. Topal¹, N. Hasanoglu Akbulut², D. Udum³, M. Yalcın⁴, O. Eyigor¹ | ¹Department of Histology and Embryology, Bursa Uludag University, Faculty of Medicine, Bursa, Türkiye; ²Department of Histology and Embryology, Canakkale Onsekiz, Mart University, Faculty of Medicine, Canakkale, Türkiye; ³Department of Biochemistry, Bursa Uludag University, Faculty of Veterinary Medicine, Bursa, Türkiye; ⁴Department of Veterinary Physiology, Bursa Uludag University, Faculty of Veterinary Medicine, Bursa, Türkiye

  https://doi.org/10.4081/ejh.2025.4349

  179

---

• THE CHEMISTRY OF NUTRACEUTICALS: DRIVING HEALTH INNOVATIONS AND SHAPING NEW PARADIGMS M. Micucci | Department of Biomolecular Sciences, Urbino University “Carlo Bo”, Urbino, Italy

  https://doi.org/10.4081/ejh.2025.4317

  232

---

• FILAMIN A IN BREAST CANCER: FROM LITERATURE TO FUNCTIONAL INSIGHTS P. Zawadka¹, W. Arendt¹, M. Gagat^1,2, M. Izdebska¹ | ¹Department of Histology and Embryology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland. ²Faculty of Medicine, Collegium Medicum, Mazovian Academy in Płock, Płock, Poland

  https://doi.org/10.4081/ejh.2025.4278

  182

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• P63 | ROLE OF ION CYCLOTRON RESONANCE ON BODY ELECTRICAL IMPEDANCE IN CHRONIC PAIN: CLINICAL EFFECTS AND FUTURE PERSPECTIVES L. Prosperi¹, G. Barassi1, M. Panunzio², P. Spaggiari³, P.E. Gallenga¹ | ¹Center for Physiotherapy, Rehabilitation and Re-Education (Ce.Fi.R.R.), Venue “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; ²Responsible Research Hospital, Campobasso, Italy; ³Scientific Association of Integrated Biochemical and Biophysical Medicine (A.M.B.B.), Mantova, Italy

  https://doi.org/10.4081/ejh.2025.4388

  619

---

• P30 | NEW INSIGHT IN THE IN VITRO-GENERATION OF INSULIN-PRODUCING CELLS: VAV1 AS A REGULATOR OF PDX1 AND MIR-375 LEVELS DURING DIFFERENTIATION OF HIPSCS INTO Β-CELLS M. Pierantoni¹, F. Brugnoli¹, V. Zamarian², M. Dell’Aira¹, V. Sordi², V. Bertagnolo¹, S. Grassilli³ | ¹Department of Translational Medicine, University of Ferrara, Ferrara, Italy; ²Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy; ³Department of Environmental and Prevention Sciences and LTTA Centre, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4350

  172

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• NANOVESICLES IN THE GUT–BRAIN AXIS: INSIGHTS FROM PROBIOTIC SUPPLEMENTATION IN IRRITABLE BOWEL SYNDROME C. Caruso Bavisotto, F. Cappello | Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, Palermo, Italy

  https://doi.org/10.4081/ejh.2025.4318

  314

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• FROM SEEING TO BELIEVING: A 43-YEAR LONG JOURNEY IN IMMUNOSTAINING G. Cattoretti | UNIMIB Pathology, Department of Medicine and Surgery, University of Milano-Bicocca, Milano, Italy

  https://doi.org/10.4081/ejh.2025.4279

  187

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• P64 | EX VIVO EXTRACTION OF GLIOMA SECRETOME: A NOVEL MICROENVIRONMENT-BASED PROTOCOL FOR METABOLIC AND MOLECULAR PROFILING I. Neri¹, M.V. Marvi¹, V. Righi², V. Papa³, E. Boschetti¹, S. Blando¹, E. Franceschi⁴, M. Zoli⁴, D. Mazzatenta⁴, N. Neri⁴, A. Vignaroli⁴, L. Cocco¹, L. Manzoli¹, S. Ratti¹ | ¹Cellular Signalling Laboratory, Anatomy Center, Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy; ²Department of Life Quality Sciences, University of Bologna, Bologna, Italy; ³IRCCS St. Orsola, Bologna, Italy; ⁴IRCCS Institute of Neurological Sciences of Bologna, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4389

  317

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• P31 | MORPHOLOGICAL AND ULTRASTRUCTURAL MARKERS OF RADIATION-INDUCED ADIPOSE TISSUE DAMAGE E. Guerra¹, S. Lattanzio¹, G. Furini², G. Scabia², E. Mota-Silva², M. Maffei², S. Cinti³, R. Di Pietro¹ | ¹Department of Medicine and Ageing Sciences, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; ²CNR Institute of Clinical Physiology, Pisa, Italy; ³Department of Experimental and Clinical Medicine, Center of Obesity, Università Politecnica delle Marche (Polytechnic University of Marche), Ancona, Italy

  https://doi.org/10.4081/ejh.2025.4351

  191

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• EXPLORING THE CYTOTOXIC AND APOPTOTIC ACTIVITIES OF PRUNUS SPINOSA ECOTYPE EXTRACT TRIGNO ON 3D AND 2D MODELS OF HUMAN CANCER CELLS A. Di Netta¹, A. Di Pauli², R. Vona³, C. Cittadini³, S. Meschini¹, M. Condello¹ | ¹ National Center for Drug Research and Evaluation, National Institute of Health, Rome, Italy; ² Department of Anatomy, Histology, Forensic-Medicine and Orthopedics, «Sapienza» University of Rome, Rome, Italy: ³ National Center for Gender-Specific Medicine, National Institute of Health, Rome, Italy

  https://doi.org/10.4081/ejh.2025.4319

  297

---

• PATHOMICS – A NOVEL OMICS APPROACH FOR HISTOPATHOLOGY P. Boor^1,2 | ¹Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany; Electron Microscopy Facility, RWTH Aachen University Hospital, Aachen, Germany; ²Division of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany

  https://doi.org/10.4081/ejh.2025.4284

  190

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• P65 | (YS) INVESTIGATING THE EFFECTS OF THE SECRETOME FROM HUMAN AMNIOTIC MESENCHYMAL STROMAL CELLS ON INFLAMMASOME ACTIVATION M. Rossi¹, M. Magatti², P. Romele², E. Vertua², E. Giuzzi², S. Farigu², L. Marelli², B. Rapuzzi², S. De Munari², A. Silini², O. Parolini³ | ¹Department of Life Science and Public Health, Università Cattolica del Sacro Cuore, Rome, and Centro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, Italy; ²Centro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, Italy; ³Department of Life Science and Public Health, Università Cattolica del Sacro Cuore, Rome, Italy & Fondazione IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo (FG), Italy

  https://doi.org/10.4081/ejh.2025.4390

  263

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• P32 | BLOCKING CANCER CELL MOVEMENT: THE ROLE OF PAXILLIN AND EZRIN IN CERVICAL TUMOR M. Hałas-Wiśniewska¹, W. Arendt¹, M. Gagat^1,2, M. Izdebska¹ | ¹Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Poland; ²Faculty of Medicine, Collegium Medicum, Mazovian Academy in Płock, Poland

  https://doi.org/10.4081/ejh.2025.4352

  181

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• BIOLOGICAL RESPONSE OF TREATMENT WITH SAFFRON PETAL EXTRACT ON CYTOKYNE-INDUCED OXIDATIVE STRESS AND INFLAMMATION IN THE CACO-2/THP1 COCOLTURE MODEL V. Panella¹, F. De Cecco², S. Franceschelli¹, M. A. Maggi³, S. Bisti⁴, A. Bravo Nuevo⁵, D. D’Ardes¹, F. Cipollone¹, L. Speranza¹ | ¹Department of Medicine and Aging Sciences, University «G. D’Annunzio», Chieti; ²Italian Institute of Technology, Genoa, Italy; ³Hortus Novus, L’Aquila, Italy; ⁴National Institute of Biostructure and Biosystem, Rome, Italy; ⁵Department of Biomedical Sciences, Philadelphia College of Osteopathic Medicine (PCOM), Philadelphia, USA

  https://doi.org/10.4081/ejh.2025.4320

  286

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• ACCELERATING DISCOVERY TOGETHER: A COMMUNITY APPROACH TO ANTIBODY VALIDATION AND MULTIPLEXED IMAGING A. Radtke | Leica Microsystems

  https://doi.org/10.4081/ejh.2025.4285

  177

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• P66 | FROM INNERVATION TO STEROIDOGENIC DEFECT: IMPLICATIONS OF CGRP IN THE PATHOPHYSIOLOGY OF POLYCYSTIC OVARY SYNDROME L. Sallicandro¹, R. Gentile², B. Filová³, S. Polakovičová³, A. Biagini¹, F. Ballarino⁴, D. Del Bianco⁴, I. Varga⁵, A. Malvasi⁶, B. Fioretti² | ¹Department of Medicine and Surgery, University of Perugia, Perugia, Italy; ²Department of Chemistry, Biology and Biotechnologies, University of Perugia, Italy; ³Department of Histology and Embriology, Comenius University, Bratislava, Slovakia; ⁴Department of Chemistry, Biology and Biotechnologies, University of Perugia, Italy; ⁵Department of Histology and Embriology, Comenius University, Bratislava, Slovakia; ⁶Department of Biomedical Sciences and Human Oncology, Obstetrics and Gynecology Unit, University of Bari Aldo Moro, Bari, Italy

  https://doi.org/10.4081/ejh.2025.4391

  324

---

• P33 | IMPACT OF CYCLIN C EXPRESSION ON THE AGGRESSIVENESS OF A549 NON-SMALL CELL LUNG CANCER CELLS V. Havryliuk | Department of Histology and Embryology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Poland

  https://doi.org/10.4081/ejh.2025.4353

  189

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• P01 | PUTATIVE EFFECTS OF THE HYDROGEL-STEM CELL-MELATONIN COMBINATION WITH OR WITHOUT A SCAFFOLD TO TREAT THIOACETAMIDE-INDUCED LIVER DEGENERATION IN RAT G. Ozgun¹, D. Yucel², M. Kolgazi³, S. Ozer⁴, N. Tozun⁵, S. Arbak⁶ | ¹Dept of Medical Biotechnology, Institute of health sciences, Acibadem Univ, Istanbul, Türkiye; ²Dept. of Histology and Embryology, School of Medicine, Acibadem Univ, Istanbul, Türkiye; ³Dept. of Physiology, School of Medicine, Acibadem Univ., Istanbul, Türkiye; ⁴Animal Application and Research Center, Acibadem Univ. Istanbul, Türkiye; ⁵Dept. of Gastroenterology, School of Medicine, Acibadem Univ., Istanbul, Türkiye; ⁶Dept. of Histology and Embryology, School of Medicine, Acibadem Univ., Istanbul, Türkiye

  https://doi.org/10.4081/ejh.2025.4321

  200

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• ANIMAL MODELS FOR LINEAGE TRACING IN (CELL) TRANSDIFFERENTIATION S. Cinti | Center for The Study of Obesity. Marche Polytechnic University, Ancona, Italy

  https://doi.org/10.4081/ejh.2025.4286

  158

---

• P67 | EMERGING ROLE OF NUCLEAR PHOSPHOLIPASE C BETA 1 AS POTENTIAL BIOMARKER IN LUNG CANCER S. Salucci¹, I. Versari¹, S. Burattini², D. Montecchio³, I. Faenza¹ | ¹Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy; ²Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, Italy; ³Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

  https://doi.org/10.4081/ejh.2025.4392

  272

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• P34 | ADJUDIN-INDUCED MODEL IN RATS REVEALS HIPPO SIGNALING PATHWAY ACTIVITY IN THE TESTIS İ. İnanç¹, O. Bender², A. Atalay², E. Erdemli¹ | ¹Ankara University, Faculty of Medicine, Department of Histology and Embryology, Ankara, Türkiye; ²Ankara University, Biotechnology Institute, Ankara, Türkiye

  https://doi.org/10.4081/ejh.2025.4354

  177

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• P02 | THE C-C CHEMOKINE-RECEPTOR 2 (CCR2)/CCL2 AXIS PARTICIPATE IN PLATELET FUNCTION IN MYELOFIBROSIS F. Arciprete¹, V. Velardi¹, G. Pozzi², C. Carubbi³, S. Cortellazzi³, A.M. Vannucchi⁴, M. Vitale⁵, R.A. Rana¹, G. Vivacqua¹, E. Masselli³, M. Zingariello¹ | ¹Unit of Microscopic and Ultrastructural Anatomy, University Campus Bio-Medico, Rome, Italy; ²Anatomy Unit, Department of Medicine and Surgery (DiMeC), University of Parma, Parma, Italy; ³Anatomy Unit, Department of Medicine and Surgery (DiMeC), University of Parma, Parma, Italy; ⁴Center Research and Innovation of Myeloproliferative Neoplasm, University Hospital Careggi, University of Florence, Florence, Italy; ⁵San Raffaele University, Milano, Italy

  https://doi.org/10.4081/ejh.2025.4322

  220

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• P68 | EARLY EFFECTS OF MECHANOACUSTIC FOCUSED VIBRATION (MFV) ON PRIMARY HUMAN SATELLITE CELLS ISOLATED FROM YOUNG AND OLDER INDIVIDUALS S. Sancilio¹, G. Stati¹, E.S. Di Filippo², S. Fulle², R. Di Pietro¹ | ¹Department of Medicine and Aging Sciences, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; ²Department of Neuroscience Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy

  https://doi.org/10.4081/ejh.2025.4393

  251

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• P35 | EARLY LIFE STRESS CAN CAUSE CHANGES IN THE HIPPO SIGNALING PATHWAY IN RAT OVARIES İ. İnanç¹, E. Baysal¹, O. Bender², A. Atalay², E. Erdemli¹ | ¹Ankara University, Faculty of Medicine, Department of Histology and Embryology, Ankara, Türkiye; ²Ankara University, Biotechnology Institute, Ankara, Türkiye

  https://doi.org/10.4081/ejh.2025.4355

  162

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• P03 | THE ROLE OF FILAMIN A IN REGULATING BREAST CANCER CELL BEHAVIOR AND TUMOR MICROENVIRONMENT INTERACTIONS W. Arendt¹, P. Zawadka¹, M. Hałas-Wiśniewska¹, M. Gagat², M. Izdebska¹ | ¹ Department of Histology and Embryology, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń, Poland; ²Department of Histology and Embryology, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruń; Faculty of Medicine, Collegium Medicum, Mazovian Academy in Płock, Poland

  https://doi.org/10.4081/ejh.2025.4323

  182

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• ANIMAL MODELS OF METASTATIC CANCER - ADVANCED IMAGE ANALYSIS FOR DISSECTION OF DRIVING SIGNALING PATHWAYS S. Alberti | University of Messina, Messina, Italy

  https://doi.org/10.4081/ejh.2025.4287

  179

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• P69 | MICROPLASTICS AND ATHEROSCLEROSIS: EVIDENCE OF MICROPLASTICS IN CAROTID ATHEROSCLEROTIC PLAQUES R. Sciarrillo¹, V. Gallicchio¹, A. Lallo¹, F. Carrella¹, D. Barbarisi², A. Falzarano¹, M. De Falco³, F. Flora², A. Peluso², S. Piccolo², L. Flora² | ¹Department of Science and Technologies, University of Sannio, Italy; ²Vascular Surgery, Hospital of National Importance San Giuseppe Moscati, Italy; ³Department of Biology, University of Naples, Italy

  https://doi.org/10.4081/ejh.2025.4394

  367

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• P36 | BIOLOGICAL ROLE OF DNA G-QUADRUPLEX DURING LIVER REGENERATION AFTER PARTIAL HEPATECTOMY T. Ishizuka, K. M. Aung, T. Kubota, B. Lkham-Erdene, K. Kai, Y. Hishikawa | Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

  https://doi.org/10.4081/ejh.2025.4358

  171

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• P04 | CYTOCHEMICAL AND BIOLOGICAL EFFECT OF BIORESONANCE IN IRRITABLE BOWEL SYNDROME G. Barassi¹, M. Panunzio², P.E. Gallenga¹ | ¹Center for Physiotherapy, Rehabilitation and Re-Education (Ce.Fi.R.R.), Venue “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; ²Responsible Research Hospital, Campobasso, Italy

  https://doi.org/10.4081/ejh.2025.4324

  242

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• THE USE OF BIOMIMETIC 2D AND 3D UROTHELIAL IN VITRO MODELS WITH MOLECULAR, MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF NORMAL AND CANCEROUS BLADDER TISSUE AS AN ALTERNATIVE METHOD IN PRECLINICAL STUDIES M.E. Kreft¹, L. Tratnjek¹, A. Janev¹, N. Resnik¹, U. Cerkvenik¹, U. D. Jerman¹, G. Markovič¹, T.Ž. Ramuta¹, T. Višnjar¹, Ž. Sardoč¹, A.Punčuh¹, S. Kralj², M. Čemažar³, P. Veranič¹ |¹Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; ²Department for Materials Synthesis, Jožef Stefan Institute, Ljubljana, Slovenia; ³Institute of Oncology Ljubljana, Ljubljana, Slovenia

  https://doi.org/10.4081/ejh.2025.4288

  221

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• P70 | TRICHOSANTHIN TARGETS WNT/NF-KB PATHWAYS TO ATTENUATE PSORIATIC INFLAMMATION AND KERATINOCYTE HYPERPROLIFERATION K. Wang¹, W. Li¹, Z. Ying¹, C. Li², O. Sha¹ | ¹Department of Anatomy and Histology, School of Basic Medical Sciences, Shenzhen University Medical School, Shenzhen, China; ²Department of Anatomy, Shantou University Medical College, Shantou, China

  https://doi.org/10.4081/ejh.2025.4395

  297

---

• P37 | THE ROLE OF ACTIN-BINDING PROTEINS IN ENDOMETRIOSIS W. Arendt¹, M. Hałas-Wiśniewska¹, M. Gagat², M. Izdebska¹ | ¹Department of Histology and Embryology, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland; ²Department of Histology and Embryology, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland; Faculty of Medicine, Collegium Medicum, Mazovian Academy in Płock, Poland

  https://doi.org/10.4081/ejh.2025.4359

  194

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• P05 | ENTERIC NERVOUS SYSTEM NEUROPLASTICITY IN COLON OF ANIMAL MODELS OF OBESITY V. Bellitto¹, I. Martinelli², G. Nittari², S.K. Tayebati², D. Tomassoni¹ | ¹School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy; ²School of Medicinal and Health Products Sciences, University of Camerino, Camerino, Italy

  https://doi.org/10.4081/ejh.2025.4325

  166

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• FROM BULK TO SELECTIVE: A JOURNEY THROUGH THE PHYSIOLOGICAL RELEVANCE OF AUTOPHAGY C. Settembre^1,2 | ¹ University of Naples Federico II, Naples, Italy; ²Telethon Institute of Genetics and Medicine (TIGEM), Naples, Italy

  https://doi.org/10.4081/ejh.2025.4290

  168

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• P71 | THE EXTRACELLULAR VESICLE POPULATIONS UNDERWENT ALTERATIONS SUBSEQUENT TO THE PRETREATMENT OF ADIPOSE-DERIVED STEM CELLS WITH MELATONIN RECEPTOR AGONIST B. Sikora, K. Pogoda-Mieszczak, E. Bogunia, A. Skubis-Sikora, P. Czekaj | Department of Cytophysiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Poland

  https://doi.org/10.4081/ejh.2025.4396

  282

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• P38 | THE ROLE OF SPEXIN IN COLORECTAL CANCER J. Kiezun¹, J. Godlewski¹, M. Kiezun², M. Gudelska¹, K. Kisielewska¹, A. E. Kowalczyk¹, B. E. Krazinski¹ | ¹Department of Anatomy and Histology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Poland; ²Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland

  https://doi.org/10.4081/ejh.2025.4360

  194

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• P06 | MICROGRAVITY-INDUCED CHANGES IN CYTOSKELETON STRUCTURE OF TCAM-2 CELLS M. Berardini¹, L. Gesualdi¹, A. Di Pauli¹, M. Signore², S. Broccolucci¹, F. Ferranti³, M. Bizzarri⁴, M. Tafani⁴, G. Ricci⁵, A. Catizone¹ | ¹Dept of Anatomy, Histology, Forensic-Medicine and Orthopedics,“Sapienza” University of Rome, Rome, Italy; ²RPPA Unit, Proteomics Area, Core Facilities, Istituto Superiore di Sanità, Rome, Italy; ³Human Spaceflight and Scientific Research Unit, Italian Space Agency, Rome, Italy; ⁴Dept of Experimental Medicine “Sapienza” University of Rome, Rome, Italy; ⁵Dept of Experimental Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Naples, Italy

  https://doi.org/10.4081/ejh.2025.4326

  189

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• ROLE OF MITOCHONDRIAL METABOLISM AND CONTACT SITES IN CANCER CELL DEATH AND SURVIVAL F. Severin, A. Ruzza, F. Vianello, E. Zaltron, I. Celotti, M. Scavezzon, S. Toffanin, C. Bastianello, L. Leanza | Department of Biology, University of Padova, Padova, Italy

  https://doi.org/10.4081/ejh.2025.4291

  238

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• P72 | A SWAB NEGATIVE PATIENT AFTER SARS-COV-2 INFECTION DISPLAYS INCREASE OF VEGF AND FIBRONECTIN ASSOCIATED WITH ALTERATIONS OF INTERCELLULAR JUNCTIONS C. Simioni¹, J.M. Sanz², R. Gafà³, G. Cenacchi⁴, S. Occhionorelli³, R. Rizzo⁵, D. Bortolotti⁵, A. Passaro³, L.M. Neri³ | ¹Department of Life Sciences and Biotechnology, University of Ferrara, Italy; ²Department of Chemical, Pharmaceutical and Agricultural, Sciences, University of Ferrara, Italy; ³Department of Translational Medicine, University of Ferrara, Italy; ⁴Department of Biomedical and Neuromotor Sciences, Alma Mater Studiorum University of Bologna, Italy; ⁵Department of Environmental and Prevention Sciences, University of Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4397

  285

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• P40 | VOLUME ELECTRON MICROSCOPY REVEALS SPECIFIC FEATURES OF MITOTIC CELLS IN THE PINEAL ORGAN OF THE DOMESTIC TURKEY B. Lewczuk, N. Szyryńska, M. Prusik | Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

  https://doi.org/10.4081/ejh.2025.4362

  173

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• P07 | DISRUPTED GUT-VASCULAR BARRIER AND INFLAMMATORY REMODELING IN MNGIE: A SPATIAL TRANSCRIPTOMIC AND HISTOLOGICAL STUDY S. Blando¹, E. Boschetti¹, I. Neri¹, L. Caporali², C. Fiorini², D. Ormanbekova², C. Malagelada³, I. Rusciano¹, V. Carelli², R. De Giorgio⁴, L. Manzoli¹, S. Ratti¹ | ¹Center of Clinical Surgical Molecular and Experimental Anatomy Alma Mater Studiorum, University of Bologna, Italy; ²IRCCS Institute of Neurological Sciences of Bologna, Neurogenetics Program, Bologna, Italy; ³Departament de Medicina, Universitat Autonoma de Barcelona, Barcelona, Spain; ⁴Department of Translational Medicine, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4327

  167

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• THE EFFECT OF MUTANT HUNTINGTIN ON CLEAVAGE OF CREST BY CALPAIN-2 AND ITS CYTOTOXITY Y. Zhang, T. Peng, H. Li | Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China

  https://doi.org/10.4081/ejh.2025.4292

  175

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• P73 | ANALYSIS OF SAPK/JNK PATHWAY IN DOXORUBICIN INDUCED ADIPOSE-DERIVED STEM CELL CYTOTOXICITY A. Skubis-Sikora, B. Sikora, K. Pogoda-Mieszczak, E. Bogunia, P. Czekaj | Department of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Poland

  https://doi.org/10.4081/ejh.2025.4398

  278

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• P41 | INVOLVEMENT OF THE DOLPHIN’S MELON IN IMMUNE FUNCTION G.P. Lombardo¹, A. Miller², L. Spiccia¹, V. Natale¹, F. Pellicanò¹, G. Squadrito¹, E. R. Lauriano¹ | ¹Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina; ²Department of Veterinary Sciences, University of Messina, Messina, Italy

  https://doi.org/10.4081/ejh.2025.4363

  202

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• P08 | COMBINED EFFECTS OF NONYLPHENOL AND STEROID HORMONES ON HUMAN PROSTATE CELL LINE S. Boccia¹, A. Mileo², F. Carrella³, R. Sciarrillo⁴, A. Capaldo¹, M. De Falco⁵ | ¹Dept. of Biology, University Federico II of Naples, Naples, Italy; ²Dept. of Medicine and Health Sciences, University of Molise, Campobasso, Italy; ³Dept. of Science and Technologies, University of Sannio, Benevento, Italy; ⁴Dept. of Science and Technologies, University of Sannio, Benevento, Italy; ⁵Dept. of Biology, University Federico II of Naples,; National Institute of Biostructures and Biosystems (INBB), Rome, Italy

  https://doi.org/10.4081/ejh.2025.4328

  182

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• HACAT CELLS AS A 2D EXPERIMENTAL DIFFERENTIATING MODEL TO ANALYZE THE EARLY BIOLOGICAL EFFECTS INDUCED BY A PSORIATIC PROINFLAMMATORY MICROENVIRONMENT: CELL PROLIFERATION, DIFFERENTIATION AND FERROPTOSIS F. Riva¹, S. Recalcati², E. Gammella², D. Daluiso¹, F. Prignano³, E. Donetti² | ¹Dept. Public Health, Experimental and Forensic Medicine; Histology and Embryology Unit, University of Pavia, Pavia, Italy; ²Dept. Biomedical Sciences for Health, University of Milan, Milan, Italy; ³Dept. Health Sciences, Section of Dermatology, University of Florence, Florence, Italy

  https://doi.org/10.4081/ejh.2025.4293

  189

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• P74 | EXPRESSION OF PISCIDIN, TLR-4, ERK2 AND P-P38 MAPK IN THE ZEBRAFISH GUT EXPOSED TO PCB-126 AND POSSIBLE PROTECTIVE EFFECTS OF GINESTEIN L. Spiccia¹, A. Miller², G.P. Lombardo¹, V. Natale¹, F. Pellicanò¹, S. Marino¹, A. Nunnari¹, A. Migliorato³, E.R. Laurian¹ | ¹Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy; ²Department of Veterinary Sciences, University of Messina, Messina, Italy; ³Department of Biomedical and Dental Sciences and Morphofunctional Images, University of Messina, Messina, Italy

  https://doi.org/10.4081/ejh.2025.4399

  252

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• P42 | PANCREATIC CANCER STEM CELLS AND FUNCTIONAL CHARACTERIZATION OF MIR-216A-5P G. Fenu¹, C.G. Lison², F. Etzi³, A. Pisano³, C. Farace³, A. Sabalic³, D. Tutedde³, J.A. Marchal⁴, R. Madeddu⁵ | ¹Department of Biomedical Science, University of Sassari, 07100 Sassari, Italy; ²Instituto de Investigación Biosanitaria ibs.GRANADA, University Hospitals of Granada-University of Granada, Granada, Spain; ³Department of Biomedical Science, University of Sassari, Sassari, Italy; ⁴Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospitals of Granada-University of Granada, Granada, Spain; ⁵Department of Biomedical Science, University of Sassari, Sassari, Italy

  https://doi.org/10.4081/ejh.2025.4364

  178

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• P09 | EFFECTS OF NUTLIN-3A ACTIVITY ON 2D AND 3D RETINOBLASTOMA MODELS F. Bompan¹, R. Foschi², A. Dipinto³, F. Casciano², G. Lodi⁴, A. Sanvido⁵, P. Severi⁴, L. Giari¹, L. C. Cosenza⁵, R. Voltan², A. Romani⁴ | ¹Department of Environmental and Prevention Sciences, University of Ferrara, Ferrara, Italy; ²Department of Environmental and Prevention Sciences, University of Ferrara, Ferrara, Italy; LTTA Centre, University of Ferrara, Ferrara, Italy; ³Department of Environmental and Prevention Sciences, University of Ferrara, Ferrara, Italy; ⁴Department of Translational Medicine, University of Ferrara, Ferrara, Italy; LTTA Centre, University of Ferrara, Ferrara, Italy; ⁵Department of Translational Medicine, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4329

  184

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• FROM CLOUD TO BEDSIDE C. Tacchetti^1,2 | ¹Medical School & S.RACE (San-Raffaele Artificial Intelligence Center);Vita-Salute University San Raffaele, Milan, Italy; ²Experimental Imaging Center, IRCCS Hospital San Raffaele, Milan, Italy

  https://doi.org/10.4081/ejh.2025.4294

  202

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• P75 | MICROBIOTA-GUT-BRAIN AXIS IN AN AUTISM SPECTRUM DISORDER ANIMAL MODEL F. Sulas¹, G. Cominelli¹, G. Favero^1,2, D. Pinto^2,3, F. Rinaldi^2,3, R. Rezzani^1,2,4 | ¹Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy; ²Interdepartmental University Center of Research Adaption and Regeneration of Tissues and Organs (ARTO), University of Brescia, Brescia, Italy; ³Human Microbiome Advanced Project Institute, Milan, Italy; ⁴Italian Society for the Study of Orofacial Pain (Società Italiana Studio Dolore Orofacciale - SISDO), Brescia, Italy

  https://doi.org/10.4081/ejh.2025.4400

  316

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• P43 | NEW INSIGHTS IN THE ACTIVATION OF FERROPTOSIS PROCESS DURING LIVER FIBROSIS R. Mancinelli¹, S. Leone¹, A. Casini¹, R. Vaccaro¹, M. Tagliafierro¹, F.M. Bassi¹, E. Bocci¹, S. Vitale¹, A. Franchitto², L. Pannarale¹, P. Onori¹, E. Gaudio¹ | ¹Department of Anatomical, Histological, Forensic Medicine and Orthopaedics Sciences, Sapienza University of Rome, Italy; ²Division of Health Sciences, Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Italy

  https://doi.org/10.4081/ejh.2025.4365

  227

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• P10 | ELECTRON MICROSCOPY-ADAPTED PHTHALOCYANINE AND VON KOSSA HISTOCHEMISTRY: A POWERFUL TOOL TO STUDY AORTIC VALVE CALCIFICATION IN AD HOC MODELS AND ACTUAL DISEASE A. Bonetti, M. Contin, M. Marchini, F. Ortolani | Department of Medicine, University of Udine, Udine, Italy

  https://doi.org/10.4081/ejh.2025.4330

  168

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• USE OF HIGH-DEFINITION IMAGING FLOW CYTOMETRY AND A.I. TO ENUMERATE AND CHARACTERISE CIRCULATING TUMOUR CELLS S. Saravi¹, S. Panfilov², E. Karteris² | ¹College of Health, Medicine and Life Sciences, Brunel University of London, UK; ²College of Health, Medicine and Life Sciences, Brunel University of London, UK

  https://doi.org/10.4081/ejh.2025.4295

  173

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• P76 | PERICAPILLARY SPACES IN THE SHEEP PINEAL GLAND – 2D AND 3D ANALYSIS N. Szyrynska, B. Lewczuk | Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

  https://doi.org/10.4081/ejh.2025.4402

  327

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• P44 | CROSSTALK AMONG NEURONS, MUSCLE AND BONE: HUMAN NEUROMUSCULAR JUNCTION ON A CHIP M. Gatti^1,2, F. Beretti², M. Malenchini², M. Y. Follo¹, F. Mancarella³, F. Bonafè³, F. Gherardini⁴, T. Maraldi² | ¹Department of Biomedical and Neuromotor Science, Cellular Signalling Laboratory, University of Bologna, Italy; ²Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; ³Istituto per lo Studio dei Materiali Nanostrutturati (ISMN), National Research Council (CNR), Bologna, Italy; ⁴Department of Engineering “Enzo Ferrari”, University of Modena and Reggio Emilia, Modena, Italy

  https://doi.org/10.4081/ejh.2025.4366

  166

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• P11 | DOMINO TRANSPLANTATION IN MNGIE: IS THE LIVER A SUITABLE GRAFT? E. Boschetti¹, I. Neri¹, S. Blando¹, C. Malagelada², L. Caporali³, R. D’Angelo⁴, R. Rinaldi⁴, S. Mongiorgi¹, V. Carelli³, R. De Giorgio⁵, L. Manzoli¹, S. Ratti¹ | ¹Center of Clinical Surgical Molecular and Experimental Anatomy Alma Mater Studiorum – University of Bologna, Italy; ²Digestive System Research Unit, University Hospital Vall d’Hebron; Barcelona, Spain; ³IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy; ⁴IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy; ⁵Department of Translational Medicine, University of Ferrara, Ferrara, Italy

  https://doi.org/10.4081/ejh.2025.4331

  202

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• SUMOYLATION AS AN EARLY ADAPTIVE MECHANISM IN THE PROTEOMIC RESPONSE OF HUMAN GERM CELLS TO SIMULATED MICROGRAVITY A. Di Pauli¹, G. Ricci², M. Crescenzi³, M. A. Mariggiò⁴, C. Morabito⁴, L. Gesualdi¹, M. Berardini¹, F. Ferranti⁵, M. Signore⁶, A. Catizone¹ | ¹Department of Anatomy, Histology, Forensic-Medicine and Orthopedics, Section of Histology and Embryology, «Sapienza» University, Rome, Italy; ²Department of Experimental Medicine, University «Luigi Vanvitelli», Naples, Italy; ³Core Facilities, Italian National Institute of Health, Rome, Italy; ⁴Department of Neuroscience, Imaging and Clinical Sciences CAST, «G. d’Annunzio» University, Chieti, Italy; ⁵Human Spaceflight and Scientific Research Unit, Italian Space Agency, Rome, Italy; ⁶RPPA unit, Proteomics, Italian National Institute of Health, Rome, Italy

  https://doi.org/10.4081/ejh.2025.4296

  198

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