Guidelines for Authors
The European Journal of Histochemistry publishes the following article types:
- Articles (Original Papers)
- Brief Reports
- Technical Notes
- Views & Comments
- Letters to the Editors
Manuscript will be carefully scrutinized for evidence of plagiarism, duplication and data manipulation; in particular, images will be carefully examined for any indication of intentional improper modification.
Any suspected misconduct ends up with a quick rejection and is then reported to the US Office of Research Integrity.
Ensure that your work is written in correct English before submission. Professional copyediting can help authors improve the presentation of their work and increase its chances of being taken on by a publisher. In case you feel that your manuscript would benefit from a professional English language copyediting checking language grammar and style, you can find a reliable revision service at:
The Corresponding Author (multiple corresponding authors are not allowed) must submit the manuscript online-only through our Manuscript Submission System.
The Authors' metadata must be duly completed, by adding "contributors" when needed. Metadata are crucial for the indexing of the paper and of authors, and for attribution of copyright to ALL authors.
Attention: Any submission received for a manuscript with more than one contributor, in which only one author has been inserted in the metadata, will result in immediate rejection, asking for a correct re-submission.
The author names and affiliations inserted in the online submission system will be those which will be published in PubMed and all other indexes. The Editorial staff is therefore not responsible for eventual inaccuracies or mistakes in the affiliations inserted during the submission process.
Authors are kindly invited to suggest potential reviewers (names, affiliations and email addresses) for their manuscript, if they wish.
Preparing your manuscript
Manuscripts should be in either British or American English consistently throughout. Check for consistent spelling of names, terms, and abbreviations, including in tables and figure captions.
Each manuscript should be typewritten, double-spaced throughout; pages should be in A4 format and numbered, lines should be left numbered in continuum (10-digit numeric system). The manuscript can be submitted either as Word or PDF format.
All manuscripts must contain the following sections:
- Ethical approval
- Availability of data and material
- Competing interests
- Authors' contributions
The manuscript should be divided into: Title page; Abstract; Text; Acknowledgments; References; Tables; Figures; Tables/Figures legends.
The Title page must contain the following information: Title of the paper; full name and surname of author(s); full name, town and country of the institution(s) where the work was done; complete address (phone and fax numbers, E-mail address) of the corresponding author (please note that multiple corresponding authors are not allowed); key words (no more than seven); authors' contributions, i.e., information about the contributions of each person named as having participated in the study (role of authors and contributors); disclosures about potential conflict of interest.
The unstructured Abstract must be analytically informative.
The Text should normally be subdivided into: Introduction, Materials and Methods, Results, Discussion.
In the Introduction, which should be concise, the purpose of the research must be clearly explained.
Materials and Methods should report all information useful for repetition of the experiments. Generic and specific names should be typed in italics. When chemicals are quoted, well defined, registered names should be used following the subject index of chemical abstracts. When the text refers to enzymes, the trivial names should be given as published in Enzyme nomenclature by Academic Press, 1984. Units of measurements should be those recommended by the International Committee for the Standardization of Units of Measurements, please check this site (http://www.bipm.org/en/si/si_brochure) for Uniform Requirements.
Results should include the presentation of all experimental data. The description of the original observations must be concise, avoiding the use of both tables and graphs to illustrate the same results. An adequate statistical analysis of quantitative data should be provided.
General considerations and conclusions should be reported in the Discussion only.
Acknowledgments will be placed at the end of the text.
References should be prepared strictly according to the Vancouver style, which is present in EndNote); for details see the URL: http://www.nlm.nih.gov/bsd/uniform_requirements.html. Where available, URLs for the references should be provided directly within the Word document.
References must be numbered consecutively in the order in which they are first cited in the text, and they must be identified in the text by arabic numerals in superscript. References to personal communications and unpublished data should be incorporated in the text and not placed under the numbered References.
The legends of Tables and Figures should be informative and concise without duplicating information presented within the body of the text. Remarks such as "see comments in the text" must be avoided.
Figures: Symbols and abbreviations used in figures can be defined in the figure caption or note or within the figure itself. Please avoid the use of boldface or greater size for the characters. Please remember that in order to promote good management of the space available images must take up the least possible space without compromising clarity. The number of figures should be reasonable and justified: no more than 20% of the article. They must be numbered with Arabic numerals and placed at the end of the manuscript.
Lettering of figures must be clearly labeled. Figures with different panels have to be grouped into a plate, and panels marked with letters. Micrographs contained in the same figure should be marked with letters.
There is no additional cost for publishing color figures.
Figures should be designed using a well-known software package. After acceptance, if the submitted figures (of adequate resolution and size) will still require major changes to be properly arranged for publication, this service is charged to the authors.
When requested by the Editors, Figures and graphs must be submitted as .tif or .jpg files, with the following digital resolution, preferably saved for MacIntosh:
- Color (saved as CMYK): 300 dpi - maximum width 17 cm
- Black and white/grays: 600 dpi - maximum width 17 cm
Manuscript format for Brief Reports:
- Main text: up to 10,000 characters, spaces included (not including abstract, illustrations, and references)
- Abstract length: up to 900 characters, spaces included
- Table/Figure limits: up to 3 tables and figures
- References: up to 40 references
In case extracts (text/figures/tables) from other copyrighted works are included, the author(s) must obtain written permission from the copyright holder(s) and credit the source(s) in the article, for example: 'Adapted from Malatesta et al., Eur J Histochem 2009;53:e12; with permission.' The editorial office of European Journal of Histochemistry needs to receive a copy of the written permission before proceeding with publication. Please download here the 'License and Disclaimer' agreement.
The author names and affiliations inserted in the online submission system are those that will be published in PubMed. The Editorial staff is therefore not responsible for eventual inaccuracies or mistakes inserted during the submission process.
All manuscripts submitted to our journal are critically assessed by external and/or in-house experts in accordance with the principles of peer review, which is fundamental to the scientific publication process and the dissemination of sound science. The first step of manuscript selection takes place entirely in-house and has two major objectives: i) to establish the article appropriateness for the readership of our journal; ii) to define the manuscript priority ranking relative to other manuscripts under consideration, since the number of papers that the journal receives is much greater than it can publish. If a manuscript does not receive a sufficiently high priority score to warrant publication, the editors will proceed to a quick rejection. The remaining articles are reviewed by at least two different external referees (second step or classical peer review). Manuscripts should be prepared according to the Uniform Requirements established by the International Committee of Medical Journal Editors (ICMJE).
Authorship and Contributorship
All persons designated as authors should qualify for authorship according to the ICMJE criteria. Each author should have participated sufficiently in the work to take public responsibility for the content. Authorship credit should only be based on substantial contributions to: i) conception and design, or analysis and interpretation of data, and to ii) drafting the article or revising it critically for important intellectual content; and on iii) final approval of the version to be published; and iv) agreement to be accountable for all aspects of the work. Participation solely in the acquisition of funding or the collection of data does not justify authorship. General supervision of the research group is not sufficient for authorship. Authors should provide a brief description of their individual contributions. Those who do not meet all four criteria should not be listed as authors, but they should be acknowledged. Those whose contributions do not justify authorship may be acknowledged individually or together as a group under a single heading. Authors can find detailed information on the Publisher's web site.
Changes in Authorship
If authors request removal or addition of an author after manuscript submission or during the peer-review process, the journal editors should receive a letter clearly explaining the reason for the change. Authors are also requested to sign and send to the Editors a statement of agreement for the requested change from all listed authors and from the author to be removed or added.
Please note that if your manuscript is accepted you will not be able to make any changes to the authors, or order of authors, of your manuscript once the editor has accepted your manuscript for publication.
No changes to the Authors or Corresponding Author can be made after publication of the article, either as an “Advance Online Article” or in the regular issue. Instead, a corrigendum may be considered by the journal editor.
Obligation to Register Clinical Trials
The ICMJE believes that it is important to foster a comprehensive, publicly available database of clinical trials. The ICMJE defines a clinical trial as any research project that prospectively assigns human subjects to intervention or concurrent comparison or control groups to study the cause-and-effect relationship between a medical intervention and a health outcome. Medical interventions include drugs, surgical procedures, devices, behavioral treatments, process-of-care changes, etc. Our journals require, as a condition of consideration for publication, registration in a public trials registry. The journal considers a trial for publication only if it has been registered before the enrollment of the first patient. The journal does not advocate one particular registry, but requires authors to register their trial in a registry that meets several criteria. The registry must be accessible to the public at no charge. It must be open to all prospective registrants and managed by a non-profit organization. There must be a mechanism to ensure the validity of the registration data, and the registry should be electronically searchable. An acceptable registry must include a minimum of data elements (http://www.icmje.org/about-icmje/faqs/clinical-trials-registration/). For example, ClinicalTrials.gov (http://www.clinicaltrials.gov), sponsored by the United States National Library of Medicine, meets these requirements.
Protection of Human Subjects and Animals in Research
When reporting experiments on human subjects, authors should indicate whether the procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2013. If doubt exists whether the research was conducted in accordance with the Helsinki Declaration, the authors must explain the rationale for their approach and demonstrate that the institutional review body explicitly approved the doubtful aspects of the study. An Informed Consent statement is always required from patients involved in any experiments. When reporting experiments on animals, authors should indicate whether the institutional and national guide for the care and use of laboratory animals was followed. Further guidance on animal research ethics is available from the World Medical Association (2016 revision). When reporting experiments on ecosystems involving non-native species, Authors are bound to ensure compliance with the institutional and national guide for the preservation of native biodiversity.