P81 | AN IN VITRO CYTOTOXIC, PROAPOPTOTIC AND ANTITUMOR ACTIVITY ASSESSMENT FOR CERAMIDASE INHIBITORS ON HUMAN LARYNGEAL CANCER CELLS

C. Vejselova Sezer¹, M. Cengiz², B. Gür³, R. Dündar⁴, C. Cingi⁵, H.M. Kutlu⁶ | ¹Department of Biology, Kutahya Dumlupinar University, Kutahya, Türkiye; ²Department of Mathematics and Physical Sciences Education, Siirt University, Siirt, Türkiye; ³Department of Biochemistry, Iğdır University, Iğdır, Türkiye; ⁴Department of Otorhinolaryngology, Acibadem Eskişehir Hospital, Eskişehir, Türkiye; ⁵Department of Otorhinolaryngology, Eskişehir Osmangazi University, Eskişehir, Türkiye; ⁶Department of Biology, Eskişehir Technical University, Eskişehir, Türkiye

Laryngeal cancer is diagnosed approximately 20% of head and neck cancers worldwide¹. Among the potential sphingolipid-based treatment methods for the treatment of these fatal cancers² acid ceramidase inhibition is indicated as a beneficial treatment option³. In this study, the cytotoxic, proapoptotic and antitumor effects of recently developed human acid ceramidase inhibitors ceranib-2 and carmofur were investigated in human laryngeal cancer cells using MTT, Annexin V and colony formation assay. It was found that ceranib-2 and carmofur caused cytotoxicity in monotherapy and in combination at low doses. Proapoptotic effect was detected both in monotherapy and in combination therapy at the IC50 concentrations (54.18, 51.94 and 27.15 µM for carmofur, ceranib-2 and their combination, respectively) after 24 h of exposure. The highest apoptosis rate was detected after the combination treatment. Inhibition of colony formation, which indicates antitumor effect, was most clearly seen in the combination group, and a significant decrease in colony number was also recorded in the monotherapy groups.
This study was supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Grant Number 123Z615. The authors thank to TUBITAK for their supports.