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Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

Gianluca Accogli, Giovanni Scillitani, Donatella Mentino, Salvatore Desantis
  • Gianluca Accogli
    University of Bari Aldo Moro, Department of Emergency and Organ Transplants, Italy
  • Giovanni Scillitani
    University of Bari Aldo Moro, Department of Emergency and Organ Transplants, Italy
  • Donatella Mentino
    University of Bari Aldo Moro, Department of Biology, Italy

Abstract

The Octopus vulgaris farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared Octopus vulgaris paralarvae during the first month of life. Four types of mucous cells were distinguished:  mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked suphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the Octopus vulgaris paralarvae survival. 

Keywords

Cephalopods; aquaculture; glycoproteins; histochemistry; lectins; skin.

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Submitted: 2017-05-30 17:19:00
Published: 2017-09-12 15:14:46
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Copyright (c) 2017 Gianluca Accogli, Giovanni Scillitani, Donatella Mentino, Salvatore Desantis

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