Brief Reports
Vol. 60 No. 2 (2016)
The knee joint loose body as a source of viable autologous human chondrocytes
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 24 March 2016
Accepted: 5 May 2016
Accepted: 5 May 2016
2511
Views
687
Downloads
394
HTML
Authors
Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.
Downloads
Download data is not yet available.
Citations
Supporting Agencies
NHMRC Project Grant 352562How to Cite
1.
Melrose J. The knee joint loose body as a source of viable autologous human chondrocytes. Eur J Histochem [Internet]. 2016 Jun. 10 [cited 2026 Jan. 28];60(2). Available from: https://www.ejh.it/ejh/article/view/2645
