An improved method for preparation of mouse retinal cryosections

Submitted: 22 June 2020
Accepted: 24 August 2020
Published: 3 September 2020
Abstract Views: 1556
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Supplementary: 145
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Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images. Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.

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Supporting Agencies

National Natural Science Foundation of China, National Precision Medicine Project

How to Cite

Li, L., Lin, Y., Yang, F., Zou, T. ., Yang, J., & Zhang, H. (2020). An improved method for preparation of mouse retinal cryosections. European Journal of Histochemistry, 64(3). https://doi.org/10.4081/ejh.2020.3154