https://doi.org/10.4081/ejh.2021.3228

HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum

Vol. 65 No. 2 (2021)
Submitted: 14 February 2021
Accepted: 13 April 2021
Published: 28 April 2021
Condylomata acuminata, Znic transporters 1, Human papillomavirus, Immunohistochemical staining
Abstract Views: 820
PDF: 499
HTML: 10
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Li Niu
https://orcid.org/0000-0003-0627-1963
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Xiaoying Chu
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Yaofei Jiang
https://orcid.org/0000-0003-3825-8288
Hubei Cancer Clinical Study Centre and Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China.
Wei Zeng
zengweixh@126.com
https://orcid.org/0000-0001-7170-4003
Hubei Cancer Clinical Study Centre and Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China.

Condyloma acuminata (CA) are benign anogenital warts caused by human papillomavirus (HPV) infection with a high recurrence rate. Despite its high contagiousness, high recurrence rate and potential for malignant transformation, effective treatments for CA have not yet been developed. Accordingly, it is necessary to clarify the mechanisms underlying CA development. Zinc (Zn) is stably maintained in the weight of human body. Skin is the third most Zn-abundant tissue in the body. Zn is present as a divalent ion (Zn2+) in cells and does not need a redox reaction upon crossing the cellular membrane. Zn transporters (ZnTs; SLC30A) and Irt-like proteins (ZIPs; SLC39A) are involved in Zn2+ efflux and uptake, respectively. ZnT1 is one of the ZnTs, which associates with the development of HPV. However, the role of ZnT1 regulation in the CA caused by HPV infection remains unknown. A multigroup case-control study was designed to investigate the expression and significance of the ZnT1 in patients with CA infected with HPV and in normal vulva controls. ZnT1 was assessed by immunohistochemistry in 44 patients with CA at Zhongnan Hospital of Wuhan University 2019-2020. Samples were analyzed by paraffin embedding and sectioning and hematoxylin-eosin and immunohistochemical staining. Immunohistochemical methods detected specific, dark brown, positive staining of ZnT1 in the keratinocytes of epidermis. We verified that the expression levels of ZnT1 that interact with HPV were upregulated in the CA groups independently of genotype compared with the control group. And then we found that the HPV risk grade in CA patients has a certain correlation with ZnT1 expression. These findings showed that HPV infection upregulated the expression of ZnT1 in CA. Additionally, there were obvious differences in the expression of ZnT1 between the different HPV risk grade infection groups. The higher the HPV risk grade, the stronger the ZNT1 protein expression. This study provided new insights into the sign pathway to HPV infection.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC
Wiraguna A, Andriani PI, Adiguna MS. Comparison of plasma zinc levels among HIV+ and HIV- subjects infected with Condyloma acuminata. Asian Pac J Cancer Prev 2019;20:943-9. DOI: https://doi.org/10.31557/APJCP.2019.20.3.943
Yin G, Li X, Wang X, Zhang H. Effect of 5-aminolevulinic acid photodynamic therapy on the expression of apoptosis inhibitors Bcl-2 and survivin in keratinocytes of condyloma acuminatum. Photodiagnosis Photodyn Ther 2019;28:53-7. DOI: https://doi.org/10.1016/j.pdpdt.2019.07.018
Paver EC, Currie AM, Gupta R, Dahlstrom JE. Human papilloma virus related squamous cell carcinomas of the head and neck: diagnosis, clinical implications and detection of HPV. Pathology 2020;52:179-91. DOI: https://doi.org/10.1016/j.pathol.2019.10.008
Nadal LR, Saad SS, Lopes Filho GJ, Joaquim HPG, Manzione TDS, Manzione CR, et al. Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata. Rev Col Bras Cir 2020;47:e20202543. DOI: https://doi.org/10.1590/0100-6991e-20202543
Drabina J, Huras H, Basta T, Posadzka E, Hosiawa W, Jablonski M, et al. [Prevalence of HPV DNA among male sexual partners of women diagnosed with CIN and early invasive cervical cancer].[Article in Polish]. Przegl Lek 2015;72:611-5.
Hammer A, de Koning MN, Blaakaer J, Steiniche T, Doorbar J, Griffin H, et al. Whole tissue cervical mapping of HPV infection: Molecular evidence for focal latent HPV infection in humans. Papillomavirus Res 2019;7:82-7. DOI: https://doi.org/10.1016/j.pvr.2019.02.004
Le Poole C, Denman CJ, Arbiser JL. Immunosuppression may be present within condyloma acuminata. J Am Acad Dermatol 2008;59:967-74. DOI: https://doi.org/10.1016/j.jaad.2008.08.011
Magallon J, Chiem K, Tran T, Ramirez MS, Jimenez V, Tolmasky ME. Restoration of susceptibility to amikacin by 8-hydroxyquinoline analogs complexed to zinc. PLoS One 2019;14:e0217602. DOI: https://doi.org/10.1371/journal.pone.0217602
Delacoux E, Evstigneeff T, Leclercq M, Rettori MC, Delons S, Naret C, et al. Skin disorders and vitamin A metabolism disturbances in chronic dialysis patients: the role of zinc, retinol-binding protein, retinol and retinoic acid. Clin Chim Acta 1984;137:283-9. DOI: https://doi.org/10.1016/0009-8981(84)90116-5
Dhaliwal S, Nguyen M, Vaughn AR, Notay M, Chambers CJ, Sivamani RK. Effects of zinc supplementation on inflammatory skin diseases: A systematic review of the clinical evidence. Am J Clin Dermatol 2020;21:21-39. DOI: https://doi.org/10.1007/s40257-019-00484-0
Lazarczyk M, Cassonnet P, Pons C, Jacob Y, Favre M. The EVER proteins as a natural barrier against papillomaviruses: a new insight into the pathogenesis of human papillomavirus infections. Microbiol Mol Biol Rev 2009;73:348-70. DOI: https://doi.org/10.1128/MMBR.00033-08
Wang X, Zhao Y, Zou X, Wang L. Relationship of P16 and Ki67 in recurrence of HPV infection and cervical intraepithelial neoplasia. Int J Clin Exp Pathol 2020;13:3174-80.
Zito Marino F, Sabetta R, Pagliuca F, Brunelli M, Aquino G, Personà S, et al. Discrepancy of p16 immunohistochemical expression and HPV RNA in penile cancer. A multiplex in situ hybridization/ immunohistochemistry approach study. Infect Agent Cancer 2021;16:22. DOI: https://doi.org/10.1186/s13027-021-00361-8
Skoczynski M, Gozdzicka-Jozefiak A, Kwasniewska A. Co-occurrence of human papillomavirus (HPV) in newborns and their parents. BMC Infect Dis 2019;19:930. DOI: https://doi.org/10.1186/s12879-019-4503-4
Sichero L, Picconi MA, Villa LL. The contribution of Latin American research to HPV epidemiology and natural history knowledge. Braz J Med Biol Res 2020;53:e9560. DOI: https://doi.org/10.1590/1414-431x20199560
Farzaneh F, Nadji SA, Khosravi D, Hosseini MS, Hashemi Bahremani M, Chehrazi M, et al. Lack of HPV in benign and malignant epithelial ovarian tumors in Iran. Asian Pac J Cancer Prev 2017;18:1233-6.
Kazlouskaya M, Fiadorchanka N. Regression of giant condyloma acuminata after one dose of 9-valent human papillomavirus (HPV) vaccine. Int J Dermatol 2019;58:e245-e7. DOI: https://doi.org/10.1111/ijd.14580
Hinten F, Hilbrands LB, Meeuwis KAP, IntHout J, Quint WGV, Hoitsma AJ, et al. Reactivation of latent HPV infections after renal transplantation. Am J Transplant 2017;17:1563-73. DOI: https://doi.org/10.1111/ajt.14181
Al-Eitan LN, Tarkhan AH, Alghamdi MA, Al-Qarqaz FA, Al-Kofahi HS. Transcriptome analysis of HPV-induced warts and healthy skin in humans. BMC Med Genomics 2020;13:35. DOI: https://doi.org/10.1186/s12920-020-0700-7
Infante-Cossio P, Gonzalo DH, Hernandez-Gutierrez J, Borrero-Martin JJ. Oral inverted ductal papilloma associated with condyloma acuminata and HPV in an HIV+ patient. Int J Oral Maxillofac Surg 2008;37:1159-61. DOI: https://doi.org/10.1016/j.ijom.2008.07.016
Hultin E, Arroyo Muhr LS, Lagheden C, Dillner J. HPV transcription in skin tumors. PLoS One 2019;14:e0217942. DOI: https://doi.org/10.1371/journal.pone.0217942
Tommasino M. HPV and skin carcinogenesis. Papillomavirus Res 2019;7:129-31. DOI: https://doi.org/10.1016/j.pvr.2019.04.003
Gross G, Ikenberg H, Petry KU, Pfister H, Schneede P, Schofer H, et al. [Condyloma acuminata and other HPV-associated diseases of the genitals, anus and urethra].[Article in German]. Hautarzt 2007;58:179-86.
Bushara O, Miller D, Giubellino A, Schomaker M, Thyagarajan B, Nelson AC. Diagnosis of epidermodysplasia verruciformis: Two cases highlighting the role of direct HPV L1 gene sequencing. J Cutan Pathol 2019;46:436-41. DOI: https://doi.org/10.1111/cup.13444
Pohthipornthawat N, Feldman S, Granter SR, Laga AC, Crum CP, Herfs M. Epidermodysplasia Verruciformis-like HPV Infection of the Vulva in Immunosuppressed Women. Int J Gynecol Pathol 2018;37:233-8. DOI: https://doi.org/10.1097/PGP.0000000000000417
Patel T, Morrison LK, Rady P, Tyring S. Epidermodysplasia verruciformis and susceptibility to HPV. Dis Markers 2010;29:199-206. DOI: https://doi.org/10.1155/2010/345436
Arnold AW, Hofbauer GF. Human papillomavirus and squamous cell cancer of the skin--epidermodysplasia verruciformis-associated human papillomavirus revisited. Curr Probl Dermatol 2012;43:49-56. DOI: https://doi.org/10.1159/000335151
Lam YW, Trinkle-Mulcahy L, Lamond AI. The nucleolus. J Cell Sci 2005;118:1335-7. DOI: https://doi.org/10.1242/jcs.01736
Eom SJ, Kim EY, Lee JE, Kang HJ, Shim J, Kim SU, et al. Zn(2+) induces stimulation of the c-Jun N-terminal kinase signaling pathway through phosphoinositide 3-kinase. Mol Pharmacol 2001;59:981-6. DOI: https://doi.org/10.1124/mol.59.5.981

Supporting Agencies

National Natural Science Foundation of China, Zhongnan Hospital of Wuhan University
Yaofei Jiang, Hubei Cancer Clinical Study Centre and Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan

Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China

Wei Zeng, Hubei Cancer Clinical Study Centre and Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan

Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China

How to Cite

Niu, L., Chu, X., Jiang, Y., & Zeng, W. (2021). HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum. European Journal of Histochemistry, 65(2). https://doi.org/10.4081/ejh.2021.3228

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.