Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-α

Submitted: 21 March 2011
Accepted: 21 June 2011
Published: 4 November 2011
Abstract Views: 1200
PDF: 576
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonealy) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-α) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

M. Markelic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
K. Velickovic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
I. Golic, University of Belgrade
Faculty of Biology, Centre for Electron Microscopy
V. Otasevic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Stancic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Jankovic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
M. Vucetic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
B. Buzadzic, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
B. Korac, University of Belgrade
Institute for Biological Research “Sinisa Stankovic"
A. Korac, Faculty of Biology
Faculty of Biology, Centre for Electron Microscopy

How to Cite

Markelic, M., Velickovic, K., Golic, I., Otasevic, V., Stancic, A., Jankovic, A., Vucetic, M., Buzadzic, B., Korac, B., & Korac, A. (2011). Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-α. European Journal of Histochemistry, 55(4), e34. https://doi.org/10.4081/ejh.2011.e34